NOV/CCN3 Antibody - BSA Free

Western Blot: NOV/CCN3 Antibody [NBP1-31416] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-31416] - Analysis of HeLa, using NBP1-31416 at 1:200 dilution.

Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-31416] - Sastric cancer N87 xenograft, using NOV antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NOV/CCN3 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human NOV/CCN3. The exact sequence is proprietary.
• Localization: Secreted
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CCN3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (83%), Bovine (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for NOV/CCN3 Antibody - BSA Free

• CCN family member 3
• CCN3
• CCN3IGF-binding protein 9
• IBP-9
• IGFBP-9
• IGFBP9novH
• Insulin-like growth factor-binding protein 9
• nephroblastoma overexpressed gene
• Nephroblastoma-overexpressed gene protein homolog
• NOV
• NOVH
• protein NOV homolog

Background

The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for NOV/CCN3 Antibody (NBP1-31416). (Showing 1 - 1 of 1 FAQ).

1. I am looking for the expression of CCN3/NOV in tumor cells and I am interested in finding an antibody that has a lot of data to support the antibody is working well. Can you make a recommendation?
   • We have two antibodies, NBP1-88154 and NBP1-88155 that have been extremely well characterized by testing at the Human Protein Atlas. Please see the Human Protein Atlas website for full testing for these products.

Bioinformatics

• Gene Symbol: CCN3
• Entrez:
  • 4856(Human)
  • 18133(Mouse)
• Uniprot:
  • P48745()