CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="NOV/CCN3 was detected in immersion fixed paraffin-embedded sections of human renal cancer tissue using Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1640-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1976-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1976-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1640-SP | |||
| Carrier Free | MAB1640-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88154-25ul | |||
1976-NV) was serially diluted and captured by Rat Anti-Mouse NOV/CCN3 Monoclonal Antibody (Catalog # MAB19762) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse NOV/CCN3 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Mouse NOV/CCN3 (Catalog # 1976-NV) was serially diluted and captured by Rat Anti-Mouse NOV/CCN3 Monoclonal Antibody (Catalog # MAB19762) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse NOV/CCN3 Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB19762-SP | |||
1640-NV) was serially diluted and captured by Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1640) coated on a Clear Polystyrene Microplate (Catalog # 
1640-NV) was serially diluted and captured by Goat Anti-Human NOV/CCN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # ![N/A NOV/CCN3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_NOV_DY1640_ELISA_1693.jpg "N/A NOV/CCN3 [Biotin]")
![N/A NOV/CCN3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_NOV_DY1976_ELISA_1743.jpg "N/A NOV/CCN3 [Biotin]")
![Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-88154] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/NOV-CCN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88154-img0013.jpg "Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-88154] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-88154] - Staining of human adrenal gland shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NOV-CCN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88154-img0016.jpg)
![Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-88154] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/NOV-CCN3-Antibody-Immunohistochemistry-Paraffin-NBP1-88154-img0015.jpg)
![Western Blot: NOV/CCN3 Antibody [NBP1-31416] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NOV-CCN3-Antibody-Western-Blot-NBP1-31416-img0015.jpg "Western Blot: NOV/CCN3 Antibody [NBP1-31416] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-31416] - Analysis of HeLa, using NBP1-31416 at 1:200 dilution.](http://images.novusbio.com/fullsize/NOV-CCN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31416-img0008.jpg)
![Immunohistochemistry-Paraffin: NOV/CCN3 Antibody [NBP1-31416] - Sastric cancer N87 xenograft, using NOV antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/NOV-CCN3-Antibody-Immunohistochemistry-Paraffin-NBP1-31416-img0009.jpg)
![Western Blot: NOV/CCN3 Antibody (HL2416) - Azide and BSA Free [NBP3-25401] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody [HL2416] (NBP3-25401) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25401_rabbit-nov-ccn3-mab-hl2416-azide-and-bsa-free-2120249371575.jpg "Western Blot: NOV/CCN3 Antibody (HL2416) - Azide and BSA Free [NBP3-25401] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CCN3 antibody [HL2416] (NBP3-25401) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: NOV/CCN3 Antibody (HL2416) - Azide and BSA Free [NBP3-25401] - CCN3 antibody [HL2416] detects secreted CCN3 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat lung. CCN3 stained by CCN3 antibody [HL2416] (NBP3-25401) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25401_rabbit-nov-ccn3-mab-hl2416-azide-and-bsa-free-2120249413365.jpg)
