Western Blot: Notch-4 Antibody [NB100-93551] - Analysis of Notch-4 in 1. Jurkat 2. U937 3. WEHI 4. Raw267 cell lysates.
Immunocytochemistry/ Immunofluorescence: Notch-4 Antibody [NB100-93551] - Notch-4 antibody (1:500) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry-Paraffin: Notch-4 Antibody [NB100-93551] - Analysis of Notch-4 in human intestine.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Notch-4 Antibody
neurogenic locus notch homolog protein 4
Notch homolog 4
Neurogenic locus notch homolog protein 4 (Notch4) is a member of the Notch family. The Notch4 protein has an extracellular domain that contains several epidermal growth factor-like (EGF) repeats and an intracellular domain that contains a multitude of domain types. The Notch4 signaling network is an evolutionarily conserved intracellular pathway that regulates physical cell to cell interactions. The Notch4 protein, once cleaved from the trans-Golgi network, is presented on the cell surface as a heterodimer. Notch4 regulates cell-fate determination as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1. In Drosophila, once these ligands are activated via the release of the notch intracellular domain (NICD), Notch4 forms a transcriptional activator complex with RBPJ/RBPSUH which then activates the genes of the Enhancer of Split (E(spl)) locus. Notch4 also plays a role in vascular, renal and hepatic development, the implementation of differentiation, proliferation and apoptotic programs, and the regulation of branching morphogenesis in the development of the vascular system. Notch4 is highly expressed in heart tissue, with moderate to low expression seen in lung, placenta, liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. Notch4 was not seen expressed in adult brain or peripheral blood leukocytes. Polymorphisms in the Notch4 gene are strongly associated with susceptibility to schizophrenia by altering either the efficiency or alternate splicing of Notch4 transcripts (PMID: 22488909). (NCBI, Uniprot, Novusbio.com)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Notch-4 Antibody (NB100-93551). (Showing 1 - 1 of 1 FAQs).
I have ordered NB100-93551. What is the positive control for the antibody?
Here are some cell lines that have good expression of this protein: Protein Atlas. I would recommend using something with strong expression as your positive control. From the site, HeLa, MCF7, NTERA2, Hep-G2, etc. all have strong expression of the protein and therefore would be good controls. These are all very common cell lines and ones we use in testing frequently.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Notch-4 Antibody and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.