NOSIP Antibody

Images

 
Western Blot: NOSIP Antibody [NBP2-19566] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: NOSIP Antibody [NBP2-19566] - Analysis of paraformaldehyde-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NOSIP Antibody [NBP2-19566] - U87 xenograft, using NOSIP antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: NOSIP Antibody [NBP2-19566] - Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: NOSIP Antibody [NBP2-19566] - Human colon carcinoma. NOSIP antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NOSIP Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human NOSIP. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOSIP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NOSIP Antibody

  • CGI-25
  • eNOS interacting protein
  • eNOS-interacting protein
  • nitric oxide synthase interacting protein
  • nitric oxide synthase-interacting protein

Background

Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NOSIP
Uniprot