Nope/IGDCC4 Antibody (2516A) [Unconjugated]

Western blot shows lysates of human uterus tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Nope at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

HEK293 human embryonic kidney cell line transfected with either (A) human Nope or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

A549 human cell line was stained with Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345, filled histogram) or Rabbit IgG control antibody (Catalog # MAB1050, open histogram) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Nope/IGDCC4 was detected in immersion fixed paraffin-embedded sections of human placenta tissue using Rabbit Anti-Human Nope/IGDCC4 Monoclonal Antibody (Catalog # MAB10345) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 2516A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Nope/IGDCC4 Antibody (2516A) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Nope/IGDCC4
  Met1-Thr957
  Accession # Q8TDY8
• Specificity: Detects human Nope/IGDCC4 in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nope/IGDCC4 Antibody (2516A) [Unconjugated]

• DDM36
• hDDM36
• IGDCC4
• immunoglobulin superfamily DCC subclass member 4
• immunoglobulin superfamily, DCC subclass, member 4
• KIAA1628
• likely ortholog of mouse neighbor of Punc E11
• LOC57722
• Neighbor of punc e11
• Nope
• NOPEFLJ42051
• Protein DDM36

Background

IGDCC4/Nope is a type I transmembrane protein that belongs to the immunoglobulin superfamily. It is expressed mostly in embryonic muscle tissues and in developing and adult nervous systems. The structural similarities between IGDCC4 and the guidance receptor of the DCC family suggest that IGDCC4 may have similar functions as the DCC family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q8TDY8()