| Reactivity | HuSpecies Glossary |
| Applications | Flow |
| Clone | 2516A |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Alexa Fluor 488 |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB10345U-100UG | |||
| Alexa Fluor 405 | FAB10345V-100UG | |||
| Alexa Fluor 594 | FAB10345T-100UG | |||
| Alexa Fluor 647 | FAB10345R-100UG | |||
| Alexa Fluor 700 | FAB10345N-100UG | |||
| Alexa Fluor 750 | FAB10345S-100UG | |||
| Unconjugated | MAB10345-100 | |||
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Chinese hamster ovary cell line CHO-derived recombinant human Nope/IGDCC4 Met1-Thr957 Accession # Q8TDY8 |
| Specificity | Detects human Nope/IGDCC4 in direct ELISAs. |
| Source | N/A |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Purity Statement | Protein A or G purified from cell culture supernatant |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Protect from light. Do not freeze.
|
| Buffer | Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
![IGDCC3 Antibody (920038) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IGDCC3 Antibody (920038) [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | FAB10345U | |
| Alexa Fluor 405 | FAB10345V | |
| Alexa Fluor 488 | FAB10345G | |
| Alexa Fluor 594 | FAB10345T | |
| Alexa Fluor 647 | FAB10345R | |
| Alexa Fluor 700 | FAB10345N | |
| Alexa Fluor 750 | FAB10345S |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Uniprot |
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![Western Blot: Neogenin Antibody [NBP1-89651] - Analysis in human cell lines A-549 and HeLa using anti-NEO1 antibody. Corresponding NEO1 RNA-seq data are presented for the same cell lines. Loading control: anti-PARP1.](http://images.novusbio.com/images2/Neogenin-Antibody-Western-Blot-NBP1-89651-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Neogenin Antibody [NBP1-89651] - Staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane. Antibody staining shown in green.](http://images.novusbio.com/images2/Neogenin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89651-img0007.jpg)
![Immunohistochemistry-Paraffin: Neogenin Antibody [NBP1-89651] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/images2/Neogenin-Antibody-Immunohistochemistry-Paraffin-NBP1-89651-img0017.jpg)
![Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a perivascular lesion, along with infiltrating cells and VSOP (blue). Image collected and cropped by CiteAb from the following publication (http://asn.sagepub.com/lookup/doi/10.1042/AN20120081), licensed under a CC-BY licence.](http://images.novusbio.com/images2/Fibronectin-Antibody-Western-Blot-NBP1-91258-img0020.jpg)
![Immunocytochemistry/Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Fibronectin at 2 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91258-img0012.jpg)
![Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.](http://images.novusbio.com/images2/Fibronectin-Antibody-Immunohistochemistry-Frozen-NBP1-91258-img0019.jpg)
![Western Blot: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis using GPC3 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), SK-N-SH (4), PC-12 (5), F9 (6)and Mouse liver (7) cell lysate.](http://images.novusbio.com/images2/Glypican-3-Antibody-(9C2)-Western-Blot-NBP2-37483-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of HeLa cells using GPC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/images2/Glypican-3-Antibody-(9C2)-Immunocytochemistry-Immunofluorescence-NBP2-37483-img0004.jpg)
![Immunohistochemistry-Paraffin: Glypican 3 Antibody (9C2) [NBP2-37483] - Analysis of breast cancer tissues using GPC3 mouse mAb with DAB staining.](http://images.novusbio.com/images2/Glypican-3-Antibody-(9C2)-Immunohistochemistry-NBP2-37483-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRIM26 Antibody [NBP2-47594] - Staining human cell line A-431 shows positivity in cytoplasm and nucleus but excluded from the nucleoli.](http://images.novusbio.com/images2/TRIM26-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47594-img0001.jpg)
![Immunohistochemistry-Paraffin: TRIM26 Antibody [NBP2-47594] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center and non-germinal center cells.](http://images.novusbio.com/images2/TRIM26 Antibody-Immunohistochemistry-Paraffin-NBP2-47594-img0003.jpg)
![Western Blot: SCYL1BP1 Antibody (4F11) [NBP2-45912] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SCYL1BP1.](http://images.novusbio.com/images2/SCYL1BP1 Antibody (4F11)-Western Blot-NBP2-45912-img0010.jpg)
![Immunohistochemistry: SCYL1BP1 Antibody (4F11) [NBP2-45912] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/images2/SCYL1BP1-Antibody-(4F11)-Immunohistochemistry-NBP2-45912-img0009.jpg)
![Immunohistochemistry: SCYL1BP1 Antibody (4F11) [NBP2-45912] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/images2/SCYL1BP1-Antibody-(4F11)-Immunohistochemistry-NBP2-45912-img0001.jpg)
![Western Blot: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis of RSK1 on Daudi cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP2-80423, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/images2/RSK1-Antibody-(JE48-76)-Western-Blot-NBP2-80423-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: RSK1 Antibody (JE48-76) [NBP2-80423] - Staining of RSK1 p90 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET7109-83, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/images2/RSK1-Antibody-(JE48-76)-Immunocytochemistry-Immunofluorescence-NBP2-80423-img0002.jpg)
![Immunohistochemistry-Paraffin: RSK1 Antibody (JE48-76) [NBP2-80423] - Analysis in mouse colon tissue using RSK1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with dd](http://images.novusbio.com/images2/RSK1-Antibody-(JE48-76)-Immunohistochemistry-Paraffin-NBP2-80423-img0007.jpg)
![Western Blot: DEF8 Antibody (4B6) [NBP2-00574] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8.](http://images.novusbio.com/images/DEF8 Antibody (4B6)-Western Blot-NBP2-00574-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: DEF8 Antibody (4B6) [NBP2-00574] Staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8.](http://images.novusbio.com/images/DEF8 Antibody (4B6)-Immunocytochemistry-Immunofluorescence-NBP2-00574-img0009.jpg)
![Immunohistochemistry-Paraffin: DEF8 Antibody (4B6) [NBP2-00574] - Staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody.](http://images.novusbio.com/images/DEF8 Antibody (4B6)-Immunohistochemistry-Paraffin-NBP2-00574-img0008.jpg)
![Western Blot: HES-1 Antibody (OTI4H1) [NBP1-47791] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HES1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HES1 (1:500).](http://images.novusbio.com/images2/HES-1-Antibody-(OTI4H1)-Western-Blot-NBP1-47791-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: HES-1 Antibody (OTI4H1) [NBP1-47791] - HeLa cells using anti-HES1 mouse monoclonal antibody.](http://images.novusbio.com/images2/HES-1-Antibody-(OTI4H1)-Immunocytochemistry-Immunofluorescence-NBP1-47791-img0010.jpg)
![Immunohistochemistry-Paraffin: HES-1 Antibody (OTI4H1) [NBP1-47791] - Staining of paraffin-embedded liver using anti-Hes1 mouse monoclonal antibody.](http://images.novusbio.com/images2/HES-1-Antibody-(OTI4H1)-Immunohistochemistry-Paraffin-NBP1-47791-img0015.jpg)
![Western Blot: ACRBP Antibody [NBP1-85407] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/images2/ACRBP-Antibody-Western-Blot-NBP1-85407-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ACRBP Antibody [NBP1-85407] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/images2/ACRBP-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85407-img0006.jpg)
![Immunohistochemistry-Paraffin: ACRBP Antibody [NBP1-85407] - Staining in human testis and endometrium tissues using anti-ACRBP antibody. Corresponding ACRBP RNA-seq data are presented for the same tissues.](http://images.novusbio.com/images2/ACRBP-Antibody-Immunohistochemistry-Paraffin-NBP1-85407-img0010.jpg)
![Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/images2/XPG-Antibody-Western-Blot-NB100-74611-img0010.jpg)
![Immunohistochemistry: XPG Antibody [NB100-74611] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/images2/XPG-Antibody-Immunohistochemistry-NB100-74611-img0008.jpg)
![Western Blot: XPG Antibody [NB100-74611] - Detection of Human ERCC5/XPG on HeLa whole cell lysate using Nb100-74611. ERCC5/XPG was also immunoprecipitated by rabbit anti-ERCC5/XPG antibody NB100-74612.](http://images.novusbio.com/images2/XPG-Antibody-Western-Blot-NB100-74611-img0002.jpg)
![Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Analysis of a FFPE human breast carcinoma tissue section using 1:100 dilution of EpCAM/TROP1 antibody (clone AUA1) on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This EpCAM antibody generated an expected immunostaining of EpCAM (CD326) protein in the membranes of the cancer cells and the inter-cellular spaces. The tumor stroma and the stromal cells did not show EpCAM immunopositivity.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Immunohistochemistry-Paraffin-NB600-1182-img0009.jpg)
![Western Blot: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - Detection of EpCAM/CD326 in two Human mammary tumor cell lines, MDA-MB-231 (mesenchyme-like) and MCF-7 (epithelium-like). Dilution: 1:500 in PBS with 5% BSA. Secondary Ab: anti-Mouse IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Western-Blot-NB600-1182-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: EpCAM/TROP1 Antibody (AUA1) [NB600-1182] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-EpCAM/TROP1 (AUA1) conjugated to Alexa Fluor 488 [NB600-1182AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/EpCAM-TROP1-Antibody-(AUA1)-Immunocytochemistry-Immunofluorescence-NB600-1182-img0016.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [Alexa Fluor 488] [NBP2-24982] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control, red represents TLR1 antibody. TLR intracellular flow kit was used for this test.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-[Alexa-Fluor-488]-Flow-Cytometry-NBP2-24982-img0002.jpg "Flow Cytometry: Rabbit IgG Isotype Control [Alexa Fluor 488] [NBP2-24982] - Intracellular analysis of TLR1 in human PBMCs (lymphocyte-gated) using this antibody. Shaded histogram represents cells alone, green represents isotype control, red represents TLR1 antibody. TLR intracellular flow kit was used for this test.")