NONO Antibodies
We offer NONO Antibodies for use in common research applications: Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Peptide ELISA, Proximity Ligation Assay, Western Blot. Each NONO Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our NONO Antibodies can be used in a variety of model species: Canine, Human, Monkey, Mouse, Primate, Rabbit, Rat. Use the list below to choose the NONO Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our NONO polyclonal antibodies and browse our NONO monoclonal antibody catalog.
Alternate Names for NONO Antibodies
anti-NONO antibody, anti-NMT5552 kDa subunit antibody, anti-non-POU domain containing, octamer-binding antibody, anti-NRB54non-POU-domain-containing, octamer-binding antibody, anti-p54(nrb) antibody
13 Results for "nono" in Primary Antibodies
![Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0023.jpg "Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review")
![Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunocytochemistry-NB100-1556-img0024.jpg)
![Immunohistochemistry: NONO Antibody [NB100-1556]](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-NB100-1556-img0026.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also immunoprecipitated using anti-NONO antibody NB100-1554 or NB100-1555 at 3 microg/mg lysate.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0018.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-1556-img0020.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Affinity purified rabbit anti-NONO used at a dilution of 1:1,000 (0.2 ug/ml).](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-1556-img0025.jpg)
![Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0017.jpg "Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (4D9) [NBP2-02060] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunocytochemistry-Immunofluorescence-NBP2-02060-img0016.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0015.jpg)
![Flow Cytometry: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Flow-Cytometry-NBP2-02060-img0001.jpg)
![Western Blot: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0002.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0003.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0004.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0006.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0007.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0014.jpg)
![Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Western-Blot-NBP2-73034-img0002.jpg "Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73034-img0004.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0009.jpg)
![Flow Cytometry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-NONO antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73034-img0003.jpg)
![Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Western-Blot-NBP2-73034-img0001.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0005.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0006.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0007.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0008.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0010.jpg)
![Western Blot: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Western-Blot-NBP2-46194-img0010.jpg "Western Blot: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO.")
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0009.jpg)
![Western Blot: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Western-Blot-NBP2-46194-img0011.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0002.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0003.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0004.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0005.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0006.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Adenocarcinoma of Human ovary tissue using NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0007.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0008.jpg)
![Immunofluorescence: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunofluorescence-NBP2-46194-img0001.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241214445.jpg "Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: NONO Antibody [NBP3-38132] - Immunofluorescence analysis of NIH-3T3 cells using NONO Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-1012202411545526.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241233821.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241233879.jpg)
![Western Blot: NONO Antibody [NBP3-38132] - Western blot analysis of various lysates using NONO Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241252848.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-10122024120670.jpg)
![Immunocytochemistry/ Immunofluorescence: NONO Antibody [NBP3-38132] - Immunofluorescence analysis of C6 cells using NONO Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-1012202411572617.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Rat liver using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-10122024122415.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-1012202411535826.jpg)
![Immunocytochemistry/ Immunofluorescence: NONO Antibody [NBP3-38132] - Immunofluorescence analysis of U-2 OS cells using NONO Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241214434.jpg)
![Western Blot: NONO Antibody (0V0N8) [NBP3-16276] - Western blot analysis of extracts of various cell lines, using NONO Rabbit mAb (NBP3-16276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Western-Blot-NBP3-16276-img0006.jpg "Western Blot: NONO Antibody (0V0N8) [NBP3-16276] - Western blot analysis of extracts of various cell lines, using NONO Rabbit mAb (NBP3-16276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276] - Immunofluorescence analysis of U-2 OS cells using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunocytochemistry-Immunofluorescence-NBP3-16276-img0005.jpg)
![Western Blot: NONO Antibody (0V0N8) [NBP3-16276] - Western blot analysis of extracts of Rat liver, using NONO Rabbit mAb (NBP3-16276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Western-Blot-NBP3-16276-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276] - Immunofluorescence analysis of C6 cells using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunocytochemistry-Immunofluorescence-NBP3-16276-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276] - Immunofluorescence analysis of NIH-3T3 cells using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunocytochemistry-Immunofluorescence-NBP3-16276-img0004.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry of paraffin-embedded human esophageal cancer using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunohistochemistry-Paraffin-NBP3-16276-img0001.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-5220251242568.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511392237.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511392225.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511365167.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511402788.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-5220251215465.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NONO] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202517251948.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NONO] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202517235762.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NONO] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202517162376.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NONO] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202517235714.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NONO] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202517273573.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NONO] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202517223993.jpg)
![Western Blot: NONO Antibody [NBP2-38716] - Analysis using Anti-NONO antibody NBP2-38716 (A) shows similar pattern to independent antibody NBP2-38727 (B).](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NBP2-38716-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NONO Antibody [NBP2-38716] - Analysis using Anti-NONO antibody NBP2-38716 (A) shows similar pattern to independent antibody NBP2-38727 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NONO Antibody [NBP2-38716] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38716-img0004.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human kidney.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0009.jpg)
![Immunohistochemistry: NONO Antibody [NBP2-38716] - Staining of human heart muscle shows strong nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-NBP2-38716-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human colon, kidney, liver and testis using Anti-NONO antibody NBP2-38716 (A) shows similar protein distribution across tissues to independent antibody NBP2-38727 (B).](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0005.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human colon.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0007.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human liver.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0008.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human testis.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0010.jpg)
![Western Blot: NONO Antibody [NBP2-38727] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NBP2-38727-img0012.jpg "Western Blot: NONO Antibody [NBP2-38727] - Analysis in human cell line NTERA-2.")
![Immunocytochemistry/Immunofluorescence: NONO Antibody [NBP2-38727] - Staining of human cell line HeLa shows positivity in nucleus but excluded from the nucleoli.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38727-img0001.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human liver.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human colon, kidney, liver and testis using Anti-NONO antibody NBP2-38727 (A) shows similar protein distribution across tissues to independent antibody NBP2-38716 (B).](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0006.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human colon.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0008.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human testis.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0009.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human kidney.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0011.jpg)
![Western Blot: NONO Antibody [NB100-40789] - Analysis of NONO using this antibody at 0.1 ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-40789-img0005.jpg "Western Blot: NONO Antibody [NB100-40789] - Analysis of NONO using this antibody at 0.1 ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).")
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-40789-img0006.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-40789-img0007.jpg)
![Immunohistochemistry: NONO Antibody (8C11) [NBP3-26110] - Image of NONO Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-26220241535575.jpg "Immunohistochemistry: NONO Antibody (8C11) [NBP3-26110] - Image of NONO Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: NONO Antibody (8C11) [NBP3-26110] - Image of NONO Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-27220248573317.jpg)
![Western Blot: NONO Antibody (8C11) [NBP3-26110] - Positive Western Blot detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Ntera-2 whole cell lysate. All lanes: NONO Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-28220241135164.jpg)
![Immunocytochemistry/Immunofluorescence: NONO Antibody (8C11) [NBP3-26110] - Staining of Hela Cells with NONO Antibody (8C11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-28220241312227.jpg)
![NONO Antibody (OTI4D9) [mFluor Violet 450 SE] [NBP2-73034MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.](http://images.novusbio.com/fullsize/nbp2-73034mfv450_mouse-nono-mab-oti4d9-mfluor-violet-450-se-226202313113227.png "NONO Antibody (OTI4D9) [mFluor Violet 450 SE] [NBP2-73034MFV450] - Vial of mFluor Violet 450 conjugated antibody. mFluor Violet 450 is optimally excited at 406 nm by the Violet laser (405 nm) and has an emission maximum of 445 nm.")