non-muscle Myosin IIA Antibody

Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP3-23426] - Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Myosin IIA Heavy Chain (a.a. 1936-1950), then detected using appropriate secondary antibody conjugated to Cy3. The antibody was used in the absence (left) or presence (right) of blocking peptide.

Western Blot: non-muscle Myosin IIA Antibody [NBP3-23426] - Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

non-muscle Myosin IIA Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: non-muscle Myosin IIA synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human non-muscle heavy chain. This peptide sequence is highly conserved in rat and mouse non-muscle Myosin IIA, but is not found in non-muscle Myosin IIB and is not well conserved in non-muscle Myosin IIC. [UniProt# P35579]
• Specificity: The antibody detects a 200 kDa protein corresponding to the apparent molecular mass of non-muscle Myosin IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH9
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 200 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for non-muscle Myosin IIA Antibody

• Cellular myosin heavy chain, type A
• DFNA17
• EPSTS
• FTNSNMMHC-IIA
• MGC104539
• MHANMMHC II-a
• MYH9 variant protein
• MYH9
• Myosin heavy chain 9
• Myosin heavy chain, non-muscle IIa
• myosin, heavy chain 9, non-muscle
• myosin, heavy polypeptide 9, non-muscle
• myosin-9
• NMHC-II-A
• NMMHC II-a
• NMMHCA
• NMMHC-A
• NMMHC-IIA
• Non-muscle myosin heavy chain A
• Non-muscle myosin heavy chain IIa
• nonmuscle myosin heavy chain II-A
• non-muscle myosin heavy polypeptide 9
• Non-muscle Myosin IIA

Background

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH9