Rat nNOS ELISA Kit (Colorimetric)

ELISA: Rat nNOS ELISA Kit (Colorimetric) [NBP2-80254] - Samples were spiked with high concentrations of Rat nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat nNOS ELISA Kit (Colorimetric) [NBP2-80254] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL

Rat nNOS ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat NOS1/nNOS in samples. No significant cross-reactivity or interference between Rat NOS1/nNOS and analogues was observed.
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.97%
• Intra-Assay: CV% < 4.87%
• Spike Recovery: 85-101%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NOS1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat nNOS ELISA Kit (Colorimetric)

• bNOS
• brain
• Constitutive NOS
• EC 1.14.13.39
• IHPS1
• Neuronal NOS
• nitric oxide synthase 1 (neuronal)
• nNOS
• N-NOS
• nNOSNOS type I
• NOS
• NOS1
• Peptidyl-cysteine S-nitrosylase NOS1

Background

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for nNOS ELISA Kit (NBP2-80254). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: NOS1
• Uniprot:
  • P29476(Rat)
  • P29476(Rat)