Human nNOS ELISA Kit (Colorimetric)

ELISA: Human nNOS ELISA Kit (Colorimetric) [NBP2-80252] - Samples were spiked with high concentrations of Human nNOS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human nNOS ELISA Kit (Colorimetric) [NBP2-80252] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL

Human nNOS ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
• Standard Curve Range: 0.156-10 ng/mL
• Sensitivity: 0.094 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.85%
• Intra-Assay: CV% < 5.48%
• Spike Recovery: 86-108%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: NOS1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human nNOS ELISA Kit (Colorimetric)

• bNOS
• brain
• Constitutive NOS
• EC 1.14.13.39
• IHPS1
• Neuronal NOS
• nitric oxide synthase 1 (neuronal)
• nNOS
• N-NOS
• nNOSNOS type I
• NOS
• NOS1
• Peptidyl-cysteine S-nitrosylase NOS1

Background

NOS (nitric oxide synathase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane and at increased level of Ca2+, nNOS is trasnlocated to cytoplasm. In cytoplasm, nNOS is dephosphorylated by calcineurin which initiates NO production (2). A phosphorylation by PKA and PKC on nNOS leads down-regulation of NO production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NOS1
• Uniprot:
  • P29475()
  • P29475()