NMDAR2C Antibody (G218B5)

Western Blot: NMDAR2C Antibody (G218B5) [NBP2-29809] - Rat Brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-NMDAR2C, clone G218B5 (1:1000 dilution). Proteins were visualized using Goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates NMDAR2C (~135 kDa)

Product Discontinued

NMDAR2C Antibody (G218B5) Summary

• Immunogen: Recombinant protein with His-tag from the C-Terminus.
• Specificity: Antibody recognizes C-Terminus of NMDAR2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRIN2C
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: Rat Brain lysate was resolved by electrophoresis, transferred to PVDF and probed with anti-NMDAR2C, clone G218B5. Proteins were visualized using Goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Mouse ascites with 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for NMDAR2C Antibody (G218B5)

• glutamate [NMDA] receptor subunit epsilon-3
• glutamate receptor, ionotropic, N-methyl D-aspartate 2C
• GRIN2C
• NMDA R, NR2C Subunit
• NMDAR2C
• NMDAR2CNR2C
• N-methyl D-aspartate receptor subtype 2C
• N-methyl-D-aspartate receptor subunit 2C
• NR2C

Background

NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B or NR2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA). Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease and also epilepsy and ischemic neuronal cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR2C Antibody (NBP2-29809)(1)

Publication using NBP2-29809

• Hernandez G, Kouwenhoven WM, Poirier E et al. Dorsal raphe stimulation relays a reward signal to the ventral tegmental area via GluN2C NMDA receptors PloS one 2023-11-06 [PMID: 37930965] (WB, Rat)
  
  Details:
  Dilution 1:200 or 1:500

Bioinformatics

• Gene Symbol: GRIN2C
• Uniprot:
  • Q14957(Human)