NMDAR2B Antibody

Western Blot: NMDAR2B Antibody [NB100-41097] - (2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody [NB100-41097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Rt, Mu, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NMDAR2B Antibody Summary

• Immunogen: Peptide with sequence C-KDAHEKDDFHHLS corresponding to internal region according to NP_000825.1.
• Localization: Cytoskeleton/cytoplasm
• Predicted Species: Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GRIN2B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 2 - 4 ug/ml
• Application Notes: WB: Approx. 170 kDa band observed in rat brain lysates (calculated MW of 166 kDa band according to NP_000825.1). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28206989).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NMDAR2B Antibody

• glutamate [NMDA] receptor subunit epsilon-2
• glutamate receptor subunit epsilon-2
• glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• hNR3
• MGC142180
• NMDAR2BMGC142178
• N-methyl D-aspartate receptor subtype 2B
• N-methyl-D-aspartate receptor subunit 3
• NR2B
• NR3

Background

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDAR2B Antibody (NB100-41097)(4)

Publications using NB100-41097

• Arning L, Kraus PH, Valentin S et al. NR2A and NR2B receptor gene variations modify age at onset in Huntington disease. Neurogenetics 2005-02-01 [PMID: 15742215]
• Proud EK Examining the Emergence of Mood and Anxiety Molecular Phenotypes Resulting from Chronic Prenatal Nicotine Exposure in Cerebral Organoids Thesis 2023-01-01 (ICC/IF, Human)
• Nicola Forte, Serena Boccella, Lea Tunisi, Alba Clara Fernández-Rilo, Roberta Imperatore, Fabio Arturo Iannotti, Maria De Risi, Monica Iannotta, Fabiana Piscitelli, Raffaele Capasso, Paolo De Girolamo, Elvira De Leonibus, Sabatino Maione, Vincenzo Di Marzo, Luigia Cristino Orexin-A and endocannabinoids are involved in obesity-associated alteration of hippocampal neurogenesis, plasticity, and episodic memory in mice Nature Communications 2021-10-21 [PMID: 34675233]
• Yi H, Iida T, Liu S et al. IL-4 mediated by HSV vector suppresses morphine withdrawal response and decreases TNFa, NR2B, and pC/EBPb in the periaqueductal gray in rats. Gene Ther. 2017-03-16 [PMID: 28206989] (IHC-Fr, Rat)

Bioinformatics

• Gene Symbol: GRIN2B
• Entrez:
  • 2904(Human)
  • 14812(Mouse)
  • 24410(Rat)
• Uniprot:
  • NP_000825.1(Human)