NMDAR2B Antibody (S59-20)

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Western Blot: NMDAR2B Antibody (S59-20) [NBP1-47608] - analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-20. Load: 15 ug protein. ...read more
Immunohistochemistry-Paraffin: NMDAR2B Antibody (S59-20) [NBP1-47608] - analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-20. Tissue: backskin. Species: Mouse. Fixation: Bouins Fixative and ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
S59-20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

NMDAR2B Antibody (S59-20) Summary

Immunogen
Fusion protein amino acids 20-271 (N-terminus) of rat NR2B
Localization
Cell Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane
Specificity
Detects approx 166kDa. No cross-reactivity against NR2A.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GRIN2B
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-10 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 0.1-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
Application Notes
1 ug/ml of GluN2B/NR2B Antibody was sufficient for detection of NR2B/GluN2B in 10 ug of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Based on homology, It's predicted to detect Human and Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for NMDAR2B Antibody (S59-20)

  • glutamate [NMDA] receptor subunit epsilon-2
  • glutamate receptor subunit epsilon-2
  • glutamate receptor, ionotropic, N-methyl D-aspartate 2B
  • hNR3
  • MGC142180
  • NMDAR2BMGC142178
  • N-methyl D-aspartate receptor subtype 2B
  • N-methyl-D-aspartate receptor subunit 3
  • NR2B
  • NR3

Background

NMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA).Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease and also epilepsy and ischemic neuronal cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol GRIN2B
Entrez
Uniprot