NMDAR2A [p Tyr1325] Antibody - Azide Free

Western Blot: NMDAR2A [p Tyr1325] Antibody [NBP2-29535] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDAR phosphorylated at Tyr1325 (Control). The phosphospecificity of this labeling is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).

• Reactivity: Hu, Mu, Rt, Bv, Ca, Ha, Sh
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

NMDAR2A [p Tyr1325] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 conjugated to KLH. Accession # Q00959
• Modification: p Tyr1325
• Specificity: Specific for endogenous levels of the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
• Predicted Species: Sheep (100%), Canine (100%), Hamster (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for NMDAR2A [p Tyr1325] Antibody - Azide Free

• GluN2A
• glutamate receptor, ionotropic, N-methyl D-aspartate 2A
• hNR2A
• NMDA receptor subtype 2A
• N-methyl D-aspartate receptor subtype 2A
• N-methyl-D-aspartate receptor subunit 2A
• subunit epsilon-1

Background

The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRIN2A
• Entrez:
  • 24409(Rat)
• Uniprot:
  • Q00959(Rat)