NMDA R, NR2B Subunit [p Tyr1472] Antibody [Unconjugated]

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-NMDA NR2B subunit (Y1472). The immunolabeling is completely eliminated by treatment with lambda -PPase.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NMDA R, NR2B Subunit [p Tyr1472] Antibody [Unconjugated] Summary

• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1472 of NMDA R, NR2B subunit
• Modification: p Tyr1472
• Specificity: Human, mouse, rat ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Y1472
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GRIN2B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
• Buffer: 100 μL in 150 mM NaCl, 10 mM HEPES, 100 μg/mL BSA and 50% glycerol, pH 7.5.
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NMDA R, NR2B Subunit [p Tyr1472] Antibody [Unconjugated]

• DEE101;GluN1;Glutamate receptor;Glutamate receptor ionotropic, NMDA 1;GRIN1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;NR1
• GRIN2B
• NMDA R, NR2B Subunit
• NMDAR2B
• NR2B

Background

Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by PKC. On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.

1. Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
2. Cheung, H. et al. (2003) J. Neurochem. 86:1441.
3. Gibb, A.J. (2004) Trends Neurosci. 27:7.
4. Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NMDA R, NR2B Subunit Antibody (PPS014)(2)

Publications using PPS014

• Yan Li, John Partridge, Carissa Berger, Alberto Sepulveda-Rodriguez, Stefano Vicini, Katherine Conant Dopamine increases NMDA-stimulated calcium flux in striatopallidal neurons through a matrix metalloproteinase-dependent mechanism European Journal of Neuroscience 2018-07-09 [PMID: 26660285]
• Tsakiri N, Kimber I, Rothwell NJ, Pinteaux E Interleukin-1-induced interleukin-6 synthesis is mediated by the neutral sphingomyelinase/Src kinase pathway in neurones. Br. J. Pharmacol., 2007-12-03;153(4):775-83. 2007-12-03 [PMID: 18059318] (Western Blot, Mouse)

Review for NMDA R, NR2B Subunit Antibody (PPS014) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer Simple Western Mouse 08/16/2017

Summary

• Application: Simple Western
• Sample Tested: mouse brain lysate
• Species: Mouse
• Lot: 1471973

Comments

• Comments: This run was performed under reducing conditions using the 12-230 kDa separation system in combination with the rabbit detection system (Lysis buffer from ARY014 proteome profiler kit, protein concentration: ~0.5µg/µl).

Bioinformatics

• Gene Symbol: GRIN2B