NMDA R, NR2B Subunit [p Tyr1472] Antibody [Unconjugated]

Images

 
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR (Control). The phosphospecificty of this labeling is demonstrated by treatment with 1200 U of lambda ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NMDA R, NR2B Subunit [p Tyr1472] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y1472 of NMDA R, NR2B subunit
Modification
p Tyr1472
Specificity
Human, mouse, rat ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Y1472
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN2B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 dilution
Reviewed Applications
Read 1 Review rated 3
using
PPS014 in the following application:

Publications
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PPS014 in the following applications:

Packaging, Storage & Formulations

Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Buffer
100 μL in 150 mM NaCl, 10 mM HEPES, 100 μg/mL BSA and 50% glycerol, pH 7.5.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NMDA R, NR2B Subunit [p Tyr1472] Antibody [Unconjugated]

  • GRIN2B
  • NMDA R, NR2B Subunit
  • NMDAR2B
  • NR2B

Background

Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by PKC. On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.
  1. Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
  2. Cheung, H. et al. (2003) J. Neurochem. 86:1441.
  3. Gibb, A.J. (2004) Trends Neurosci. 27:7.
  4. Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NMDA R, NR2B Subunit Antibody (PPS014)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Review for NMDA R, NR2B Subunit Antibody (PPS014) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using PPS014:
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Simple Western
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Images Ratings Applications Species Date Details
Simple Western NMDA R, NR2B Subunit PPS014
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3
reviewed by:
Verified Customer
Simple Western Mouse 08/16/2017
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Summary

ApplicationSimple Western
Sample Testedmouse brain lysate
SpeciesMouse
Lot1471973

Comments

CommentsThis run was performed under reducing conditions using the 12-230 kDa separation system in combination with the rabbit detection system (Lysis buffer from ARY014 proteome profiler kit, protein concentration: ~0.5µg/µl).

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Verified Customer
08/16/2017
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol GRIN2B