NMDA R, NR2A Subunit Antibody [Unconjugated]

Images

 
Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-terminal NR2A-subunit of the ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NMDA R, NR2A Subunit Antibody [Unconjugated] Summary

Immunogen
Fusion protein from the C-terminus of the NR2A subunit of rat NMDA Receptor
Specificity
Specific for the ~180 kDa NR2A subunit of the NMDA Receptor in Western blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GRIN2A
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 dilution
  • Immunohistochemistry 1:1000 to 1:2000 dilution
  • Immunoprecipitation
Publications
Read Publications using
PPS012 in the following applications:

Packaging, Storage & Formulations

Storage
After reconstitution the antibody should be aliquoted and stored at ≤ -20° C. This product is stable at ≤- 20° C for at least 1 year.
Buffer
Lyophilized from 5 mM ammonium bicarbonate
Preservative
No Preservative
Reconstitution Instructions
This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NMDA R, NR2A Subunit Antibody [Unconjugated]

  • EPND
  • FESD
  • GluN2A
  • GRIN2A
  • LKS
  • NMDA R, NR2A Subunit
  • NMDAR2A
  • NR2A

Background

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes, which code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitutes the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB300-106
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IP
NB300-105
Species: Hu, Mu, Rt, Rb
Applications: WB, IHC, IHC-P, IP
NBP2-75510
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-22399
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC
NB300-124
Species: Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr
NB300-107
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP
NBP1-88191
Species: Hu, Mu
Applications: WB, IHC, IHC-P
36340002
Species: Hu
Applications: WB, ELISA
NB100-858
Species: Hu, Mu, GP
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, PEP-ELISA, IHC-WhMt
NBP1-02312
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, B/N, ICC
NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-88227
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
NLS3208
Species: Hu, Po, Bv, Eq, Mk, Pm
Applications: WB, IHC, IHC-P, ICC
NB100-65315
Species: Bv, Ca, Eq, Gt, Sh
Applications: Flow, IHC, IHC-Fr, IP
NBP2-46198
Species: Hu
Applications: WB, IHC
PPS012
Species: Hu, Mu, Rt
Applications: WB, IHC, IP

Publications for NMDA R, NR2A Subunit Antibody (PPS012)(3)

Reviews for NMDA R, NR2A Subunit Antibody (PPS012) (0)

There are no reviews for NMDA R, NR2A Subunit Antibody (PPS012). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for NMDA R, NR2A Subunit Antibody (PPS012) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional NMDA R, NR2A Subunit Products

Bioinformatics Tool for NMDA R, NR2A Subunit Antibody (PPS012)

Discover related pathways, diseases and genes to NMDA R, NR2A Subunit Antibody (PPS012). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for NMDA R, NR2A Subunit Antibody (PPS012)

Discover more about diseases related to NMDA R, NR2A Subunit Antibody (PPS012).
 

Pathways for NMDA R, NR2A Subunit Antibody (PPS012)

View related products by pathway.

PTMs for NMDA R, NR2A Subunit Antibody (PPS012)

Learn more about PTMs related to NMDA R, NR2A Subunit Antibody (PPS012).

Blogs on NMDA R, NR2A Subunit

There are no specific blogs for NMDA R, NR2A Subunit, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

nNOS Antibody
NB100-858
NMDAR2B Antibody
NB300-106-100ul

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our NMDA R, NR2A Subunit Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol GRIN2A