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Recombinant Human NM23-H2/NME2 Protein

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SDS-Page: NM23-H2/NME2 Recombinant Protein [NBP2-52251] - 15% SDS Page (3 ug)
SDS-Page: NM23-H2/NME2 Recombinant Protein [NBP2-52251] - 15% SDS Page (3 ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE, Bioactivity
Concentration
1 mg/ml

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Recombinant Human NM23-H2/NME2 Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-152 of Human NM23-H2/NME2

Source: E.coli

Amino Acid Sequence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
Details of Functionality
Specific activity is > 1,800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NME2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
17.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human NM23-H2/NME2 Protein

  • EC 2.7.13.3
  • Histidine protein kinase NDKB
  • MGC2212
  • NDK B
  • NDKB
  • NDP Kinase B2
  • NDPKB
  • NDPK-B
  • NM23B
  • NM23-H2
  • NME2
  • non-metastatic cells 2, protein (NM23B) expressed in
  • nucleoside diphosphate kinase B
  • protein (NM23) expressed in

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (1D7) [H00004830-M02] - Analysis of monoclonal antibody to NME1 on HeLa cell. Antibody concentration 10 ug/ml.
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Immunocytochemistry/Immunofluorescence: c-Myc Antibody [NB600-335] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-c-Myc conjugated to Alexa Fluor 488 [NB600-335AF488] at 20ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the Alexa Fluor 488 format of this antibody.
Western Blot: c-Myc Antibody [NB600-335] - Analysis using the HRP conjugate of NB600-335. Detection of 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.4 ug/ml (1:25,000).
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Western Blot: PUM2 Antibody [NB100-387] - Functionality of products expressed from miRNA134. 1, 2 and miRNA99a.1. miR-134 expressed from the miRNA134.1 construct affects the Pum2 protein levels. 10-11 DIV hippocampal neurons were transduced with purified rAAV carrying the indicated plasmids. Eight days later whole cell extracts were prepared and subjected to western blot analysis probing with Pum2 antibody and a beta-actin antibody as loading control. Here we show one out of three representative blots, on average displaying a significant 64% reduction in Pum2 protein levels. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/neuro.04.016.2009/abstract), licensed under a CC-BY licence.
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Recombinant Human
NM23-H2/NME2 Protein
NBP2-52251
Recombinant Human NM23-H2/NME2 Protein
Species: Hu
Applications: Func, PAGE, Bioactivity

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Bioinformatics

Gene Symbol NME2