Recombinant Human NM23-H2/NME2 Protein

SDS-Page: NM23-H2/NME2 Recombinant Protein [NBP2-52251] - 15% SDS Page (3 ug)

Product Discontinued

Recombinant Human NM23-H2/NME2 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-152 of Human NM23-H2/NME2
  
  

  Source: E.coli

  Amino Acid Sequence: MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

• Details of Functionality: Specific activity is > 1,800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NME2
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 17.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human NM23-H2/NME2 Protein

• EC 2.7.13.3
• Histidine protein kinase NDKB
• MGC2212
• NDK B
• NDKB
• NDP Kinase B2
• NDPKB
• NDPK-B
• NM23B
• NM23-H2
• NME2
• non-metastatic cells 2, protein (NM23B) expressed in
• nucleoside diphosphate kinase B
• protein (NM23) expressed in

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NME2