NM23-H2/NME2 Antibody

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human NM23-H2 Polyclonal Antibody (Catalog # AF6665) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for NM23-H2 at approximately 20 and 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

NM23-H2 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human NM23-H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6665) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NM23-H2/NME2 Antibody Summary

• Immunogen: E. coli-derived recombinant human NM23-H2
  Ala2-Glu152
  Accession # P22392
• Specificity: Detects human NM23-H2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human NM23-H1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NME2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NM23-H2/NME2 Antibody

• EC 2.7.13.3
• Histidine protein kinase NDKB
• MGC2212
• NDK B
• NDKB
• NDP Kinase B2
• NDPKB
• NDPK-B
• NM23B
• NM23-H2
• NME2
• non-metastatic cells 2, protein (NM23B) expressed in
• nucleoside diphosphate kinase B
• protein (NM23) expressed in

Background

NM23-H2 or NME-2 (non-metastatic cell 2; also Nucleoside diphosphate kinase B, nm23-H2/B, NDPK-B and PUF) is a 17-19 kDa class 1 member of the NME/NDPK family of molecules. It is widely expressed, found in both cytosol and nucleus, and participates in multiple activities. In conjunction with nm23-H1, NME-2 forms homo- and heterohexamers (three dimers or two trimers) that mediate the transfer of phosphate from ATP to nucleoside diphosphates. It also serves as a transcriptional regulator of genes such as c-myc and PDGF-A. Human NME-2 is 152 amino acids (aa) in length. It contains a kinase region (aa 5-134), two potential phosphorylation sites (Tyr52 and Thr94), a phosphohistidine residue at position 118, and multiple lysine acetylation sites. There is one potential NME-2 splice form that shows a six aa substitution for aa 77-152. The genes for NME-1 and NME-2 are adjacent, and an unusual splicing event generates a 33 kDa fusion protein (NM23-LV) that is composed of aa 1-114 of NME-1 plus a Thr insert that is N-terminally coupled to full-length NME-2. Full-length human NME-2 shares 88% and 98% aa identity with human NME-1 and mouse NME-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NME2
• Uniprot:
  • P22392()