NM23-H2/NME2 Antibody (2E3) - BSA Free

Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate

Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NM23-H2/NME2 Antibody (2E3) - BSA Free Summary

• Immunogen: Recombinant human NM23-H2/NME2 (1-152aa) purified from E. coli
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NME2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NM23-H2/NME2 Antibody (2E3) - BSA Free

• EC 2.7.13.3
• Histidine protein kinase NDKB
• MGC2212
• NDK B
• NDKB
• NDP Kinase B2
• NDPKB
• NDPK-B
• NM23B
• NM23-H2
• NME2
• non-metastatic cells 2, protein (NM23B) expressed in
• nucleoside diphosphate kinase B
• protein (NM23) expressed in

Background

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NME2