NLRP1/NALP1 Antibody (447916) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: IHC
• Clone: 447916
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

NLRP1/NALP1 Antibody (447916) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human NLRP1/NALP1
  Gly1331-Leu1429
  Accession # Q9C000
• Specificity: Detects human NLRP1/NALP1 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from cell culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for NLRP1/NALP1 Antibody (447916) [CoraFluor™ 1]

• CARD7
• CARD7SLEV1
• Caspase recruitment domain-containing protein 7
• CLR17.1
• Death effector filament-forming ced-4-like apoptosis protein
• DEFCAP
• DEFCAPVAMAS1
• DKFZp586O1822
• KIAA0926DEFCAP-L/S
• NACHT, leucine rich repeat and PYD (pyrin domain) containing 1
• NACHT, leucine rich repeat and PYD containing 1
• NACHT, LRR and PYD containing protein 1
• NACHT, LRR and PYD domains-containing protein 1
• NACPP1044
• NALP1
• NALP1caspase recruitment domain protein 7
• NLR family, pyrin domain containing 1
• NLRP1
• Nucleotide-binding domain and caspase recruitment domain
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 1
• PP1044
• SLEV1
• VAMAS1

Background

NALP1 (also known as NAC/CARD7) is a member of the CATERPILLER (CLR) diverse multigene superfamily of immune regulatory proteins (Ting and Davis, 2005). CLR proteins have a variable N-terminal domain, followed by a nucleotide-binding domain (NBD) and leucine-rich repeats (LRR). The N-terminal domain consists of transactivation, CARD, Pyrin or BIR domains, or in some cases is undefined. The CLR family, also known as the NOD family, has its ancient roots in the plant kingdom and is related to the disease resistance (R) gene family that mediates plant immune responses. CLR proteins participate in both innate and adaptive immunity in mammals, some act as sensors that detect pathogen products, and several CLR genes have been linked to susceptibility to immunological diseases. It is thought that the CLR family may have a fundamental importance for the function of the mammalian innate/ancient immune system on par with the Toll-like receptor (TLR) family. NALP1, a member of NALP CLR subfamily, has an N-terminal pyrin domain (PYD), followed by an NBD, LRR, and a C-terminal CARD domain (reviewed in Tschopp. NALP1 is a component of both the inflammasome and apoptosome, suggesting that NALP1 it has important roles in both inflammation and apoptosis. This antibody recognizes NALP1; human NALP1 is a 1473 amino acid protein and migrates at approx. 166 kDa on SDS-PAGE. However, multiple splice variants encoding distinct NALP1 isoforms with varying amino acid lengths have been described and thus the molecular weight detected may vary depending on the isoform(s) expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.