NKX2.5 Antibody (259415) [FITC]

• Reactivity: Hu
• Applications: WB
• Clone: 259415
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

NKX2.5 Antibody (259415) [FITC] Summary

• Immunogen: E. coli-derived recombinant human NKX2.5
  Gln24-Arg137
  Accession # P52952
• Specificity: Detects endogenous human NKX2.5 in Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX2-5
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Alternate Names for NKX2.5 Antibody (259415) [FITC]

• cardiac-specific homeo box
• cardiac-specific homeobox 1
• Cardiac-specific homeobox
• CHNG5
• CSX1
• CSXFLJ97195
• FLJ52202
• FLJ99536
• Homeobox protein CSX
• Homeobox protein NK-2 homolog E
• homeobox protein Nkx-2.5
• NK2 transcription factor related, locus 5 (Drosophila)
• NKX2.5
• NKX2.5FLJ97166
• NKX2-5
• NKX2E
• NKX2EFLJ97197
• NKX4-1
• tinman paralog

Background

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NKX2-5