NKp44/NCR2 Antibody (253422) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB
• Clone: 253422
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

NKp44/NCR2 Antibody (253422) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NKp44/NCR2
  Gln22-Pro190
  Accession # O95944
• Specificity: Detects human NKp44/NCR2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse MAR-1, recombinant human (rh) NKp30, rhNKp46, or rhNKp80 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp44/NCR2 Antibody (253422) [Alexa Fluor® 750]

• CD336 antigen
• CD336
• LY95
• LY95lymphocyte antigen 95 homolog (activating NK-receptor; NK-p44)
• lymphocyte antigen 95 (activating NK-receptor; NK-p44)
• Lymphocyte antigen 95 homolog
• natural cytotoxicity triggering receptor 2
• Natural killer cell p44-related protein
• NCR2
• NK cell activating receptor (NKp44)
• NK cell-activating receptor
• NKp44
• NK-p44dJ149M18.1

Background

NKp44, along with NKp30 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors" (NCR) (1). These receptors are expressed almost exclusively by NK cells and play a major role in triggering NK-mediated killing of most tumor cell lines. No rodent ortholog to NKp44 has been identified. Human NKp44, also known as NCR2, is a 44 kDa type I transmembrane glycoprotein that is characterized by the presence of one extracellular V-like immunoglobulin domain (2). It is synthesized as a 276 amino acid (aa) precursor that contains a 21 aa signal sequence, a 171 aa extracellular region, a 21 aa transmembrane segment and a 63 aa cytoplasmic tail. Alternate splicing in both the cytoplasmic tail and extracellular region generates multiple isoforms of unknown significance. The Ig-like region is unaffected. A physical association with the ITAM-bearing accessory protein, DAP12, occurs via a charged residue in the NKp44 transmembrane domain. Ligation of NKp44 with a specific antibody results in phosphorylation of DAP12 (3) and activation of target cell lysis in a redirected killing assay (4). NKp44 is absent from resting NK cells but is upregulated upon activation with IL-2. Activation-induced expression occurs in the CD56 dim CD16 + NK subset that accounts for more than 85% of NK cells found in peripheral blood and spleen, as well as the CD56 bright CD16 - NK subset that constitutes the majority of NK cells in lymph node and tonsil (5). Studies with neutralizing antibodies reveal that NKp44 is partially responsible for triggering lytic activity against several tumor cell types (2, 6). Blocking any of the individual NCRs results in partial inhibition of tumor cell lysis, but nearly complete inhibition of lysis is observed if all three receptors are blocked simultaneously (6). NKp44 has also been implicated in recognition of virus-infected cells through its capacity to bind to viral hemagglutinins (7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • O95944()