NKp30/NCR3 Antibody (210845) [Unconjugated]

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Human NKp30 Monoclonal Antibody induces IFN‑ gamma secretion in the NK-92 human natural killer lymphoma cell line in a dose-dependent manner, as measured using the Quantikine Human IFN‑ gamma ELISA Kit (Catalog # ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, Func
Clone
210845
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

NKp30/NCR3 Antibody (210845) [Unconjugated] Summary

Additional Information
Clone 210845 was used by HLDA to establish CD designation
Immunogen
Mouse T cell hybridoma transfected with human NKp30/NCR3 and Mouse myeloma cell line NS0-derived recombinant human NKp30 Fc Chimera
Specificity
Detects human NKp30/NCR3 on cell transfectants and peripheral blood NK cells.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NCR3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Agonist Activity 0.2-1.2 ug/mL
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
Publications
Read Publications using
MAB1849 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NKp30/NCR3 Antibody (210845) [Unconjugated]

  • Activating natural killer receptor p30
  • CD337 antigen
  • CD337
  • CD337activating NK-A1 receptor
  • LY117,1C7NK-p30
  • lymphocyte antigen 117
  • natural cytotoxicity triggering receptor 3
  • Natural killer cell p30-related protein
  • NCR3
  • NKp30
  • NKp30MALS

Background

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed “Natural Cytotoxicity Receptors” (1). These receptors play a major role in triggering NK‑mediated killing of most tumor cells lines. NKp30 is a type I transmembrane protein having a single extracellular V-like immunoglobulin domain (2). A physical association with the ITAM-bearing accessory protein, CD3 zeta , occurs via a charged residue in the NKp30 transmembrane domain. Ligation of NKp30 with a specific antibody results in phosphorylation of CD3 zeta (3). NKp30 is expressed on both resting and activated NK cells of the CD56dim, CD16+ subset that account for more that 85% of NK cells found in peripheral blood and spleen (4). NKp30 is absent from the CD56bright, CD16- subset that constitutes the majority of NK cells in lymph node and tonsil, however, its expression is up-regulated in these cells upon IL-2 activation (4). Studies with neutralizing antibodies reveal that NKp30 is partially responsible for triggering lytic activity against several tumor cell types and that it is the main receptor responsible for NK‑mediated lysis of immature dendritic cells (2, 5). The ligand(s) recognized by NKp30 has not been described.

  1. Moretta, L. and A. Moretta (2004) EMBO J. 23:255.
  2. Pende, D. et al. (1999) J. Exp. Med. 190:1505.
  3. Augugliaro, R. et al. (2003) Eur. J. Immunol. 33:1235.
  4. Ferlazzo, G. et al. (2004) J. Immunol. 172:1455.
  5. Ferlazzo, G. et al. (2002) J. Exp. Med. 195:343.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NKp30/NCR3 Antibody (MAB1849)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Epitope Mapping, Flow Cytometry, Neutralization.


Filter By Application
Epitope Mapping
(1)
Flow Cytometry
(3)
Neutralization
(2)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using MAB1849 Applications Species
N Kim, MY Kim, YU Cho, W Chen, KH Lee, HS Kim PVR and ICAM-1 on Blast Crisis CML Stem and Progenitor Cells with TKI Resistance Confer Susceptibility to NK Cells Cancers (Basel), 2020;12(7):. 2020 [PMID: 32708713] (Flow Cytometry, Human) Flow Cytometry Human
Z Djaoud, LA Guethlein, A Horowitz, T Azzi, N Nemat-Gorg, D Olive, D Nadal, PJ Norman, C Münz, P Parham Two alternate strategies for innate immunity to Epstein-Barr virus: One using NK cells and the other NK cells and ?? T cells J. Exp. Med., 2017;0(0):. 2017 [PMID: 28468758] (Neutralization, Human) Neutralization Human
Herrmann , Julia, Berberich , Hannah, Hartmann , Jessica, Beyer , Steffen, Davies , Karen, Koch , Joachim Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands. J Biol Chem, 2014;289(2):765-77. 2014 [PMID: 24275655] (Flow Cytometry, Human) Flow Cytometry Human
Shi J, Tricot GJ, Garg TK, Malaviarachchi PA, Szmania SM, Kellum RE, Storrie B, Mulder A, Shaughnessy JD, Barlogie B, van Rhee F Bortezomib down-regulates the cell-surface expression of HLA class I and enhances natural killer cell-mediated lysis of myeloma. Blood, 2007;111(3):1309-17. 2007 [PMID: 17947507] (Neutralization, Human) Neutralization Human
Warren HS, Warren&lt;/LastName&gt;&lt;ForeNam HS&lt;/Initia, Bettadapura J A novel binding assay to assess specificity of monoclonal antibodies. J. Immunol. Methods, 2005;305(1):33-8. 2005 [PMID: 16125193] (Epitope Mapping, Human) Epitope Mapping Human
Warren HS, Jones AL, Freeman C, Bettadapura J, Parish CR Evidence that the cellular ligand for the human NK cell activation receptor NKp30 is not a heparan sulfate glycosaminoglycan. J. Immunol., 2005;175(1):207-12. 2005 [PMID: 15972650] (Flow Cytometry, Human) Flow Cytometry Human

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Product General Protocols

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FAQs for NKp30/NCR3 Antibody (MAB1849) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB1849U
Alexa Fluor 405 FAB1849V
Alexa Fluor 488 FAB1849G
Alexa Fluor 488 FAB1849G
Alexa Fluor 532 FAB1849X
Alexa Fluor 594 FAB1849T
Alexa Fluor 647 FAB1849R
Alexa Fluor 700 FAB1849N
Alexa Fluor 750 FAB1849S
Allophycocyanin FAB1849A
Allophycocyanin FAB1849A
Biotin FAB1849B
DyLight 350 FAB1849D
DyLight 405 FAB1849E
DyLight 488 FAB1849K
DyLight 550 FAB1849L
DyLight 594 FAB1849M
DyLight 650 FAB1849W
DyLight 680 FAB1849Y
DyLight 755 FAB1849Z
FITC FAB1849F
Janelia Fluor 549 FAB1849I
Janelia Fluor 646 FAB1849J
PerCP FAB1849C
Phycoerythrin FAB1849P
Phycoerythrin FAB1849P

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Bioinformatics

Gene Symbol NCR3