NKCC2/SLC12A1 Antibody - BSA Free

Western Blot: NKCC2/SLC12A1 Antibody [NBP1-44269] - Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2/SLC12A1 protein using Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269). Primary Antibody: Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269) at 1:1000.

Immunohistochemistry: NKCC2/SLC12A1 Antibody [NBP1-44269] - Immunohistochemistry analysis using Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269). Tissue: Kidney. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NKCC2/SLC12A1 Polyclonal Antibody (NBP1-44269) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NKCC2/SLC12A1 Antibody (D) Composite

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NKCC2/SLC12A1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NKCC2/SLC12A1 Antibody - BSA Free (NBP1-44269) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
• Localization: Membrane
• Specificity: Detects approx 160kDa.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC12A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of NKCC2 Antibody was sufficient for detection of NKCC2 in 10 ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NKCC2/SLC12A1 Antibody - BSA Free

• BSC1
• Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
• Kidney-specific Na-K-Cl symporter
• MGC48843
• NKCC2
• NKCC2A variant A
• NKCC2Na-K-2Cl cotransporter
• SLC12A1
• solute carrier family 12 (sodium/potassium/chloride transporters), member 1
• solute carrier family 12 member 1

Background

NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC12A1
• Entrez:
  • 6557(Human)
  • 25065(Rat)
• Uniprot:
  • Q5PQV1(Rat)