Nitrilase 1 Antibody (1C3) - Azide and BSA Free

Western Blot: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 expression in transfected 293T cell line by NIT1 monoclonal antibody (M01), clone 1C3.Lane 1: NIT1 transfected lysate (Predicted MW: 35.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of monoclonal antibody to NIT1 on HeLa cell . Antibody concentration 10 ug/ml.

Immunoprecipitation: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Analysis of NIT1 transfected lysate using anti-NIT1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NIT1 monoclonal antibody.

Sandwich ELISA: Nitrilase 1 Antibody (1C3) [H00004817-M01] - Detection limit for recombinant GST tagged NIT1 is 1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP
• Clone: 1C3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Nitrilase 1 Antibody (1C3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NIT1 (NP_005591.1, 228 a.a. ~ 327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFGSITGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRRHLPVFQHRRPDLYGNLGHPLS
• Specificity: Reacts with nitrilase 1.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NIT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against recombinant protein in WB and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Nitrilase 1 Antibody (1C3) - Azide and BSA Free

• EC 3.5
• MGC57670
• nitrilase 1
• nitrilase homolog 1

Background

Nitrilase 1 play a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppres

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NIT1
• Entrez:
  • 4817(Human)
• Uniprot:
  • NP_005591(Human)