NIPBL Antibody

Images

 
Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-52168] - staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-52168] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

NIPBL Antibody Summary

Immunogen
Peptide with sequence C-RPDSPRVKQGDSNK corresponding to internal region according to NP_056199.2.
Epitope
C-RPDSPRVKQGDSNK
Specificity
This antibody is expected to recognize both reported isoforms (NP_597677.2; NP_056199.2).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NIPBL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as large as the calculated size of 316 kDa band according to NP_597677.2 or 304 kDa band according to NP_056199.2. IHC-P successful usage on mouse tissues and on cryosections of mouse tissues and loss of signal was observed in Nipbl-deficient cells of homozygous mutant embryos.
IHC: Paraffin embedded Human Kidney.
Publications
Read Publication using NBP1-52168.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NIPBL Antibody

  • CDLS1
  • delangin
  • DKFZp434L1319
  • FLJ11203
  • FLJ12597
  • FLJ13354
  • FLJ13648
  • FLJ44854
  • IDN3-B
  • IDN3CDLS
  • Nipped-B homolog (Drosophila)
  • nipped-B-like protein
  • SCC2 homolog
  • Scc2
  • sister chromatid cohesion 2 homolog

Background

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NIPBL Antibody (NBP1-52168)(1)


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Bioinformatics

Gene Symbol NIPBL
Entrez
Uniprot