NIPBL Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: NIPBL Antibody [NBP1-52168] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-52168] - (2ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: NIPBL Antibody [NBP1-52168] - Negative Control showing staining of paraffin embedded Human Cerebellum, with no primary antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

NIPBL Antibody Summary

Immunogen
Peptide with sequence C-RPDSPRVKQGDSNK corresponding to internal region according to NP_056199.2.
Epitope
C-RPDSPRVKQGDSNK
Specificity
This antibody is expected to recognize both reported isoforms (NP_597677.2; NP_056199.2).
Predicted Species
Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
NIPBL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
IHC: Paraffin embedded Human Kidney.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nucleus of HeLa cells.
Publications
Read Publication using NBP1-52168.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NIPBL Antibody

  • CDLS1
  • delangin
  • DKFZp434L1319
  • FLJ11203
  • FLJ12597
  • FLJ13354
  • FLJ13648
  • FLJ44854
  • IDN3-B
  • IDN3CDLS
  • Nipped-B homolog (Drosophila)
  • nipped-B-like protein
  • SCC2 homolog
  • Scc2
  • sister chromatid cohesion 2 homolog

Background

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NIPBL Antibody (NBP1-52168)(1)


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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional NIPBL Products

Array NBP1-52168

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Blogs on NIPBL

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Bioinformatics

Gene Symbol NIPBL
Entrez
Uniprot