NIPBL Antibody (3B9) - Azide and BSA Free

Western Blot: NIPBL Antibody (3B9) [H00025836-M01] - Detection against Immunogen (37.73 KDa) .

Immunocytochemistry/Immunofluorescence: NIPBL Antibody (3B9) [H00025836-M01] - Analysis of monoclonal antibody to NIPBL on HeLa cell . Antibody concentration 10 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, ChIP
• Clone: 3B9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NIPBL Antibody (3B9) - Azide and BSA Free Summary

• Immunogen: NIPBL (AAH33847, 66 a.a. ~ 175 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. FHPKQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT
• Specificity: NIPBL - Nipped-B homolog (Drosophila)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NIPBL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation Sequencing
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: Use in CHIP-SEQ, IP reported in scientific literature (PMID:34315879). Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NIPBL Antibody (3B9) - Azide and BSA Free

• CDLS1
• delangin
• DKFZp434L1319
• FLJ11203
• FLJ12597
• FLJ13354
• FLJ13648
• FLJ44854
• IDN3-B
• IDN3CDLS
• Nipped-B homolog (Drosophila)
• nipped-B-like protein
• SCC2 homolog
• Scc2
• sister chromatid cohesion 2 homolog

Background

This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and mental retardation. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NIPBL Antibody (H00025836-M01)(1)

Publication using H00025836-M01

• Garcia P, Fernandez-Hernandez R, Cuadrado A et al. Disruption of NIPBL/Scc2 in Cornelia de Lange Syndrome provokes cohesin genome-wide redistribution with an impact in the transcriptome Nature communications 2021-07-27 [PMID: 34315879] (Chip Cytometry, IP)

Bioinformatics

• Gene Symbol: NIPBL
• Entrez:
  • 25836(Human)
• OMIM:
  • 122470(Human)
• Uniprot:
  • AAH33847(Human)