NIFK Antibody (CL2237) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human cell line U-251 MG

Staining of human kidney shows strong positivity in nucleoli in cells in tubules.

Staining of human fallopian tube shows strong positivity in nucleoli in glandular cells.

Staining of U-2 OS cells using the Anti-MK167 monoclonal antibody) .

Staining of human skin shows strong positivity in nucleoli in squamous epithelial cells.

Staining of human small intestine shows strong positivity in nucleoli in glandular cells.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL2237
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NIFK Antibody (CL2237) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NIFK Antibody (CL2237) - Azide and BSA Free (NBP2-36750) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q9BYG3, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NIFK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for NIFK Antibody (CL2237) - Azide and BSA Free

• hNIFK
• MKI67 (FHA domain) interacting nucleolar phosphoprotein
• MKI67 FHA domain-interacting nucleolar phosphoprotein
• NIFKNOPP34
• Nopp34
• Nucleolar phosphoprotein Nopp34
• Nucleolar protein interacting with the FHA domain of pKI-67

Background

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NIFK