NIFK Antibody (18E148)

Images

 
Western Blot: NIFK Antibody (18E148) [NB100-56590] - Analysis using Azide Free version of NB100-56590. NIFK in 30 ugs of Ramos cell lysate using NIFK antibody at 0.5 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
18E148
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

NIFK Antibody (18E148) Summary

Immunogen
This antibody was developed against a synthetic peptide corresponding to a portion of human NIFK between amino acids 100-200.
Specificity
A 34 kDa band is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MKI67IP
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.5-3 ug/ml
Positive Control
NIFK Lysate (NBL1-13120)

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.2% gelatin
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for NIFK Antibody (18E148)

  • hNIFK
  • MKI67 (FHA domain) interacting nucleolar phosphoprotein
  • MKI67 FHA domain-interacting nucleolar phosphoprotein
  • NIFKNOPP34
  • Nopp34
  • Nucleolar phosphoprotein Nopp34
  • Nucleolar protein interacting with the FHA domain of pKI-67

Background

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NIFK Antibody (NB100-56590) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56590AF405
Alexa Fluor 488 Labeled NB100-56590AF488
Alexa Fluor 647 Labeled NB100-56590AF647
Alexa Fluor 700 Labeled NB100-56590AF700
Biotin Labeled NB100-56590B
DyLight 350 Labeled NB100-56590UV
DyLight 405 Labeled NB100-56590V
DyLight 488 Labeled NB100-56590G
DyLight 550 Labeled NB100-56590R
DyLight 650 Labeled NB100-56590C
DyLight 680 Labeled NB100-56590FR
DyLight 755 Labeled NB100-56590IR
HRP Labeled NB100-56590H

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Bioinformatics

Gene Symbol MKI67IP
Entrez
Uniprot

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