Nidogen-1/Entactin Antibody (2R33) - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2R33
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Nidogen-1/Entactin Antibody (2R33) - Azide and BSA Free Summary

• Immunogen: Human recombinant Nidogen-1/Entactin
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NID1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-2000
  • Immunohistochemistry 1:100-1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:250
  • Western Blot 1:500-1:1000
• Application Notes: This Entactin antibody (clone 2R33) is useful for Western Blot, ELISA and Immunohistochemistry-Paraffin applications. In its IHC-P validation, the antigen retrieval was performed by incubation of sections with Proteinase K (20ug/ml in PBS) for 15min at room temperature (Note: the length of PK exposure should be optimized by the user as it may depend upon the variation of fixatives and length of fixation applied to a particular specimen).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein G purified
• Reconstitution Instructions: Reconstitute with sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Nidogen-1/Entactin Antibody (2R33) - Azide and BSA Free

• enactin
• Entactin
• Entactin-1
• NID1
• NID-1
• NIDentactin
• nidogen (enactin)
• nidogen 1
• Nidogen1
• Nidogen-1

Background

Entactin encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NID1
• Uniprot:
  • NP_002499(Human)