Nidogen-1/Entactin Antibody (2R33)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2R33
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Nidogen-1/Entactin Antibody (2R33) Summary

Immunogen
This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified recombinant Nidogen-1/Entactin. Mouse anti human Nidogen-1/Entacin NP_002499.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NID1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:2000
  • Immunohistochemistry 1:50-1:250
  • Immunohistochemistry-Paraffin 1:50 - 1:250
Application Notes
This Entactin antibody (clone 2R33) is useful for Western Blot, ELISA and Immunohistochemistry-Paraffin applications. In its IHC-P validation, the antigen retrieval was performed by incubation of sections with Proteinase K (20ug/ml in PBS) for 15min at room temperature (Note: the length of PK exposure should be optimized by the user as it may depend upon the variation of fixatives and length of fixation applied to a particular specimen).

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
A 0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein G purified
Reconstitution Instructions
Reconstitute with 0.5 ml sterilized PBS to a final concentration of 0.2 mg/ml.

Alternate Names for Nidogen-1/Entactin Antibody (2R33)

  • enactin
  • Entactin
  • Entactin-1
  • NID1
  • NID-1
  • NIDentactin
  • nidogen (enactin)
  • nidogen 1
  • Nidogen1
  • Nidogen-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol NID1
Entrez
Uniprot