Nicastrin Antibody - BSA Free

Western Blot: Nicastrin Antibody [NBP1-77269] - Mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.

Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP1-77269] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) Nicastrin. Overlay images indicate the possible co-localization of VDR/Nicastrin on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Nicastrin Antibody [NBP1-77269] - Human brain tissue with Nicastrin antibody at 5 ug/ml.

Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269] - Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Nicastrin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Nicastrin Antibody - BSA Free (NBP1-77269) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Nicastrin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human Nicastrin. The immunogen is located within amino acids 630 - 680 of Nicastrin. Amino Acid Squence: TEYSTWTESRWKDIRAR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCSTN
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 0.5-2 ug/ml
• Control:

  Human Brain Frontal Lobe Whole Tissue Lysate (Adult Whole Alzheimer's)

• Control Peptide:

  Nicastrin Peptide (NBP1-77269PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Nicastrin Antibody - BSA Free

• anterior pharynx-defective 2
• APH2
• ATAG1874
• KIAA0253APH2
• NCSTN
• Nicastrin

Background

Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the gamma-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, gamma-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the alpha and gamma cleavage site) or the toxic Abeta amyloid peptide (from the beta and gamma cleavage site). It is thought that accumulation of the Abeta peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nicastrin Antibody (NBP1-77269)(1)

Publication using NBP1-77269

• Dursun E. , Gezen-Ak D. Vitamin D receptor is present on the neuronal plasma membrane and is co-localized with amyloid precursor protein, ADAM10 or Nicastrin. PLoS One 2017-11-28 [PMID: 29176823] (ICC/IF, Rat)

Bioinformatics

• Gene Symbol: NCSTN
• Uniprot:
  • NP_056146(Human)