| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5378-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77269-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5378-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB53781-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77270-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57365-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89828-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38741-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89826-50ul | |||
![Western Blot: Nicastrin Antibody [NBP1-77269] - Mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Western-Blot-NBP1-77269-img0002.jpg "Western Blot: Nicastrin Antibody [NBP1-77269] - Mouse brain tissue lysate with Nicastrin antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP1-77269] - Cell surface staining of VDR (green) on live neurons followed by fixation and immunofluorescence labeling of target protein (100x) Nicastrin. Overlay images indicate the possible co-localization of VDR/Nicastrin on the neuronal plasma membrane. Image collected and cropped by CiteAb from the following publication (//journals.plos.org/plosone/article?id=10.1371/journal.pone.0188605) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77269-img0004.jpg)
![Immunohistochemistry-Paraffin: Nicastrin Antibody [NBP1-77269] - Human brain tissue with Nicastrin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Immunohistochemistry-Paraffin-NBP1-77269-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77269] - Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77269_rabbit-polyclonal-nicastrin-antibody-2711202420175178.jpg)
![Western Blot: Nicastrin Antibody [NBP1-77270] - Human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/ml.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Western-Blot-NBP1-77270-img0002.jpg "Western Blot: Nicastrin Antibody [NBP1-77270] - Human brain tissue lysate with Nicastrin antibody at (A) 0.5 and (B) 1ug/ml.")
![Immunohistochemistry: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunohistochemistry of Nicastrin in human brain tissue with Nicastrin antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-271120241928886.jpg)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunofluorescence of Nicastrin in mouse brain tissue with Nicastrin antibody at 20 ug/ml.Green: Nicastrin Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-2711202420141926.gif)
![Immunocytochemistry/ Immunofluorescence: Nicastrin Antibody - BSA Free [NBP1-77270] - Immunofluorescence of Nicastrin in Human Brain cells with Nicastrin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77270_rabbit-polyclonal-nicastrin-antibody-2711202419333434.jpg)
![Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP2-57365] - Staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57365-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nicastrin Antibody [NBP2-57365] - Staining of human cell line U-2 OS shows localization to cytosol.")
![Western Blot: Nicastrin Antibody (019) [NBP2-89828] - Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:78 kDa Observed band size:170 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Nicastrin-Antibody-019-Western-Blot-NBP2-89828-img0001.jpg "Western Blot: Nicastrin Antibody (019) [NBP2-89828] - Lane A: Jurkat Whole Cell Lysate Lane B: A431 Whole Cell LysateLane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:78 kDa Observed band size:170 kDa (We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: Nicastrin Antibody (019) [NBP2-89828] - Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-NCSTN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-NCSTN rabbit monoclonal antibody,at 1:150 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 78 kDa Observed band size: 130 kDa](http://images.novusbio.com/fullsize/Nicastrin-Antibody-019-Immunoprecipitation-NBP2-89828-img0002.jpg)
![Immunohistochemistry: Nicastrin Antibody [NBP2-38741] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Nicastrin-Antibody-Immunohistochemistry-NBP2-38741-img0001.jpg "Immunohistochemistry: Nicastrin Antibody [NBP2-38741] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.")
![Immunoprecipitation:[NBP3-29882]-Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody used for IP at 6 µg per reaction. For blotting immunoprecipitated Nicastrin, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29882_rabbit-nicastrin-pab-231202511595015.jpg "Immunoprecipitation:[NBP3-29882]-Detection of human Nicastrin by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-Nicastrin antibody used for IP at 6 µg per reaction. For blotting immunoprecipitated Nicastrin, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: Nicastrin Overexpression Lysate (Adult Normal) [NBP2-10287] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nicastrin.](http://images.novusbio.com/fullsize/Nicastrin-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10287-img0001.jpg "Western Blot: Nicastrin Overexpression Lysate (Adult Normal) [NBP2-10287] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nicastrin.")
![Immunocytochemistry/Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826] - Staining of Human NCSTN in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89826 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89826_rabbit-monoclonal-nicastrin-antibody-004-235202514331519.png "Immunocytochemistry/Immunofluorescence: Nicastrin Antibody (004) [NBP2-89826] - Staining of Human NCSTN in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89826 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.")