NHERF-1 Antibody (EBP-10)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clone
EBP-10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NHERF-1 Antibody (EBP-10) Summary

Immunogen
Bacterially produced recombinant full-length human NHERF1.
Specificity
The antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SLC9A3R1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Immunoprecipitation 1:50
Application Notes
Western Blot: 2 ug/ml, 60 min on vertical incubator Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Non-reducing conditions. 10% separating SDS-PAGE gel.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for NHERF-1 Antibody (EBP-10)

  • EBP50NHERF-1
  • ezrin-radixin-moesin binding phosphoprotein-50
  • Ezrin-radixin-moesin-binding phosphoprotein 50
  • Na(+)/H(+) exchange regulatory cofactor NHE-RF1
  • Na+/H+ exchange regulatory co-factor
  • NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
  • NHERFNPHLOP2
  • Regulatory cofactor of Na(+)/H(+) exchanger
  • sodium/hydrogen exchanger regulatory factor 1
  • Sodium-hydrogen exchanger regulatory factor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1
  • Solute carrier family 9 isoform A3 regulatory factor 1

Background

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SLC9A3R1
Entrez