NHE3/SLC9A3 Antibody (19F5) [Biotin]

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Product Details

Summary
Reactivity Rt, Rb, Hu(-)Species Glossary
Applications WB, ELISA, ICC/IF, IHC-Fr, IHC-P
Clone
19F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

NHE3/SLC9A3 Antibody (19F5) [Biotin] Summary

Immunogen
Fusion protein representing the C-terminal 131 amino acids of rabbit NHE3. [UniProt# P26432]
Localization
Membrane; multi-pass membrane.
Specificity
This antibody is specific for NHE3. It does not show any cross-reactivity with any other NHE isoforms.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC9A3
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This NHE3 antibody is useful for Immunoprecipitation, Immunohistochemistry on frozen and paraffin-embedded sections and Western blot, where a band can be seen at ~93 kDa. Immunocytochemistry/Immunofluorescence and ELISA were reported in scientific literature.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System

Reactivity Notes

Rat and rabbit. Immunogen sequence has 83% homology to mouse. Does not react with human.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for NHE3/SLC9A3 Antibody (19F5) [Biotin]

  • isoform 3
  • MGC126718
  • NHE3
  • NHE3MGC126720
  • SLC9A3
  • solute carrier family 9 (sodium/hydrogen exchanger), member 3
  • Solute carrier family 9 member 3

Background

Na+/H+ exchanger (NHE) family members are integral membrane proteins which play key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE3 or SLC9A3 is one of the best characterized NHEs that is a major proton extruding system driven by inward Na+ ion chemical gradient and is implicated in pH regulation to eliminate acids generated during metabolism or to tackle any potential adverse conditions in tissue microenvironment. NHE3 exists as a multi-pass membrane protein in cell membranes and is expressed most abundantly in colon and small intestine, followed by kidney and stomach, as well as in ventrolateral region of the medulla oblongata. In intestinal epithelial cells, it localizes to the ileal brush border and SGK1 mediated phosphorylation at Ser-661 has been shown to be associated with its enhanced cell membrane localization. NHE3 binds to SLC9A3R1 and SLC9A3R2, and interacts with CHP1, CHP2, SHANK2 PDZD3 and (interactions may decrease in response to elevated calcium ion levels). NHE3 is regulated by several hormones, neurotransmitters, and associated signaling systems such as cAMP, cGMP, and elevated intracellular calcium via mechanisms that are largely partially understood. NHE3 knock-out mice suffers from chronic diarrhea and altered salt as well as water homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 405 Labeled NB110-61586V
DyLight 488 Labeled NB110-61586G
DyLight 550 Labeled NB110-61586R
DyLight 650 Labeled NB110-61586C
FITC Labeled NB110-61586F
HRP Labeled NB110-61586H

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Bioinformatics

Gene Symbol SLC9A3
Entrez