NHE1/SLC9A1 Antibody - BSA Free

Western Blot: NHE1/SLC9A1 Antibody [NBP1-76848] - MOLT4 cell lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76848] - Immunohistochemical staining of human brain tissue using NHE1/SLC9A1 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: NHE1/SLC9A1 Antibody - BSA Free [NBP1-76848] - Immunofluorescence of NHE1/SLC9A1 in Human Brain cells with NHE1/SLC9A1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Po, Bv, Rb
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

NHE1/SLC9A1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NHE1/SLC9A1 Antibody - BSA Free (NBP1-76848) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the center of the human Nhe-1. The immunogen is located within amino acids 490 - 540 of Nhe-1. Amino Acid Squence: AVKKKQETKRSINEEIH
• Predicted Species: Porcine (100%), Rabbit (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC9A1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  NHE1/SLC9A1 Peptide (NBP1-76848PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for NHE1/SLC9A1 Antibody - BSA Free

• APNH
• APNH1
• APNHFLJ42224
• Na(+)/H(+) antiporter, amiloride-sensitive
• Na(+)/H(+) exchanger 1
• Na+/H+, amiloride sensitive)
• Na-Li countertransporter
• NHE1
• NHE-1
• NHE1Na+/H+ antiporter, amiloride-sensitive
• SLC9A1
• sodium/hydrogen exchanger 1
• solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter
• solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter
• solute carrier family 9 (sodium/hydrogen exchanger), member 1
• Solute carrier family 9 member 1

Background

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SLC9A1
• Uniprot:
  • P19634(Human)