NGFI-B alpha/Nur77/NR4A1 Antibody (S02-1C7)

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody (S02-1C7) [NBP3-19726] - Western blot detection of NGFI-B alpha/Nur77/NR4A1 in Rat Brain lysates using NBP3-19726 (1:500 diluted). Predicted band size: 65kDa. Observed band size: 65kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S02-1C7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

NGFI-B alpha/Nur77/NR4A1 Antibody (S02-1C7) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human NGFI-B alpha/Nur77/NR4A1 (Uniprot # P22736)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NR4A1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000-1/5000
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for NGFI-B alpha/Nur77/NR4A1 Antibody (S02-1C7)

• Early response protein NAK1
• GFRP1ST-59
• growth factor-inducible nuclear protein N10
• HMR
• HMRNP10
• hormone receptor
• MGC9485
• N10
• NAK1
• NAK-1
• NGFIB alpha
• NGFI-B alpha
• NGFIB
• NR4A1
• Nuclear hormone receptor NUR/77
• nuclear receptor subfamily 4 group A member 1
• nuclear receptor subfamily 4, group A, member 1
• Nur77
• Orphan nuclear receptor HMR
• Orphan nuclear receptor TR3
• steroid receptor TR3
• Testicular receptor 3
• TR3 orphan receptor
• TR3

Background

Nak1 is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nak-1 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. Nak1 is a phosphoprotein and its size ranges from 67 kD to 88 kD, depending on post-translational modifications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR4A1