Orthogonal Strategies: Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining in human lymph node and cerebral cortex tissues using NBP1-87758 antibody. Corresponding NFKB1 RNA-seq data are ...read more
Genetic Strategies: Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in SK-BR-3 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NFKB1 antibody. Remaining ...read more
Immunocytochemistry/ Immunofluorescence: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Western Blot: NFkB p105/p50 Antibody [NBP1-87758] - Analysis in human cell line Daudi.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human colon shows moderate cytoplasmic positivity in glandular and lymphoid cells.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human lymph node shows strong cytoplasmic positivity in germinal- and non-germinal center cells.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody [NBP1-87758] - Staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: GTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHGTMDTESKKDPEGCDKSDDKNTVNLFGKVIETTEQDQEPSEATVGNGEVTLTYATGTKEESAGVQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAV
Immunogen affinity purified
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Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for NFkB p105/p50 Antibody
DNA binding factor KBF1
DNA-binding factor KBF1
nuclear factor kappa-B DNA binding subunit
nuclear factor NF-kappa-B p105 subunit
nuclear factor NF-kappa-B p50 subunit
nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151
NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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