NFATC3/NFAT4 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

NFATC3/NFAT4 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human NFATC3
  Asp900-Asp1035
  Accession # Q12968
• Specificity: Detects human NFATC3 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NFATC3/NFAT4 Antibody [Alexa Fluor® 488]

• NFAT4
• NF-AT4
• NFAT4nuclear factor of activated T-cells, cytoplasmic 3
• NFATC3
• NF-ATc3
• NFATX
• nuclear factor of activated T-cells c3 isoform IE-Xa
• nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3
• T cell transcription factor NFAT4
• T-cell transcription factor NFAT4

Background

NFATC3 (Nuclear factor of activated T cells C3; also NFAT4 and NFATx) is a 145-150 kDa member of the NFAT family of transcription factors. NFATC3 is widely expressed being found in DP thymocytes, CD4+ T cells, smooth muscle cells, Schwann cells and mast cells. NFATC3 regulates gene transcription as part of a NFATC transcription complex. It is normally cytoplasmic and phosphorylated. Upon a rise in intracellular Ca +2 dephosphorylation occurs via calcineurin, and NFATC3 enters the nucleus. Human NFATC3 is 1075 amino acids (aa) in length. It contains a calcineurin binding site (aa 109-114), two nuclear localization sites (aa 273-275 and 686-688), one nuclear export site (aa 1032-1041) and an RH domain that binds DNA (aa 433-592). There are multiple isoforms with different tissue expression profiles. Over aa 900-1035, human NFATC3 shares 93% aa identity with mouse NFATC3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q12968()