NFATC1/NFAT2 Antibody (4I9P10)

Western Blot: NFATC1/NFAT2 Antibody (4I9P10) [NBP3-15803] - Western blot analysis of extracts of various cell lines, using NFATC1/NFAT2 antibody (NBP3-15803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 4I9P10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

NFATC1/NFAT2 Antibody (4I9P10) Summary

• Description: Novus Biologicals Rabbit NFATC1/NFAT2 Antibody (4I9P10) (NBP3-15803) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 844-943 of human NFATC1/NFAT2 (O95644). PSSPSPPLPPATQEPTCLQPCSPACPPATGRPQHLPSTVRRDESPTAGPRLLPEVHEDGSPNLAPIPVTVKREPEELDQLYLDDVNEIIRNDLSSTSTHS
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFATC1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 μg/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NFATC1/NFAT2 Antibody (4I9P10)

• MGC138448
• NFAT transcription complex cytosolic component
• NFAT2
• NFAT2cytoplasmic 1
• NFATC
• NF-ATC
• NFATC1
• NF-ATc1
• nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

Background

The nuclear factor of activated T-cells (NFAT) transcription complex is required for the expression of a group of proteins that coordinate the immune response. NFAT2 is the cytosolic component of NFAT and is encoded by a separate gene. Expression of NFAT2 activates the interleukin (IL-2) promoter in non-T lymphocytes and is required for IL-2 gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NFATC1