NF-M Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NF-M knockout HeLa cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NF-M Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3029) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NF-M at approximately 155 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IHC, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NF-M Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NF-M
  Ser2-Ser358
  Accession # P07197
• Specificity: Detects human NF-M in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) NF-H and rhNF-L is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NEFM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Western Blot 1-2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NF-M Antibody [Unconjugated]

• NEF3
• NEF3neurofilament medium polypeptide
• NEFM
• Neurofilament 3
• neurofilament, medium polypeptide 150kDa
• neurofilament, medium polypeptide
• neurofilament-3 (150 kD medium)
• NFM
• NF-M
• NF-M160 kDa neurofilament protein
• NFMNeurofilament triplet M protein

Background

Human NF-M (neurofilament-medium length) is a 145‑150 kDa phosphorylated glycoprotein that belongs to the type IV intermediate filament family. It is 915 amino acids in length and contains a 100 amino acid (aa) N-terminal globular region, a 320 aa alpha -helical rod, or central region, and a 500 aa globular tail. The rod region contains a series of heptad repeats that contribute to a coiled-coil interaction with an adjacent NF-M molecule, the first step in intermediate filament oligomerization. The C-terminus shows phosphorylation and O-linked glycosylation. Human NF-M aa 2‑358, which includes most of the rod region, shares ~98% aa sequence identity with corresponding region of mouse, rat and canine NF-M.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-M Antibody (AF3029)(1)

Publication using AF3029

• Behzadi, A;Tjust, AE;Liu, JX;Andersen, PM;Br�nnstr�m, T;Pedrosa Domell�f, F; Myofiber Type Shift in Extraocular Muscles in Amyotrophic Lateral Sclerosis Investigative ophthalmology & visual science 2023-05-01 [PMID: 37200039] (IHC, Human)

Bioinformatics

• Gene Symbol: NEFM
• Uniprot:
  • P07197()