NF-M Antibody (NF-09) [Alexa Fluor® 488]

Product Discontinued

NF-M Antibody (NF-09) [Alexa Fluor® 488] Summary

• Immunogen: Pellet of pig brain cold stable proteins after depolymerization of microtubules.
• Marker: Neuronal Marker
• Specificity: The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for NF-M Antibody (NF-09) [Alexa Fluor® 488]

• NEF3
• NEF3neurofilament medium polypeptide
• NEFM
• Neurofilament 3
• neurofilament, medium polypeptide 150kDa
• neurofilament, medium polypeptide
• neurofilament-3 (150 kD medium)
• NFM
• NF-M
• NF-M160 kDa neurofilament protein
• NFMNeurofilament triplet M protein

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-M Antibody (NB500-446AF488)(1)

Publication using NB500-446AF488

• Lee H, Xu B, Lewkowicz A et al. Rvsv-Ebov-Gp Challenge Models Acute and Chronic Eye Infections with Ebola and Can Be Used to Test Therapeutics that Target the Ebola Glycoprotein papers.ssrn.com 2023-11-28 (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: NEFM
• Entrez:
  • 4741(Human)
  • 24588(Rat)