NF-M Antibody (NF-09) - BSA Free

Western Blot: NF-M Antibody (NF-09) [NB500-446] - Analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.

Immunocytochemistry/Immunofluorescence: NF-M Antibody (NF-09) [NB500-446] - Staining of neurofilament medium protein in murine Neuro2A cells by antibody NF-09 conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue).

Product Discontinued

NF-M Antibody (NF-09) - BSA Free Summary

• Immunogen: Pellet of pig brain cold stable proteins after depolymerization of microtubules.
• Marker: Neuronal Marker
• Specificity: The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1-2 ug/ml
• Application Notes: Immunocytochemistry - Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  NF-M Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for NF-M Antibody (NF-09) - BSA Free

• NEF3
• NEF3neurofilament medium polypeptide
• NEFM
• Neurofilament 3
• neurofilament, medium polypeptide 150kDa
• neurofilament, medium polypeptide
• neurofilament-3 (150 kD medium)
• NFM
• NF-M
• NF-M160 kDa neurofilament protein
• NFMNeurofilament triplet M protein

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NEFM
• Entrez:
  • 4741(Human)
  • 24588(Rat)