NF-M Antibody (NF-09)

Images

 
Western Blot: NF-M Antibody (NF-09) [NB500-446] - Analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.
Immunocytochemistry: NF-M Antibody (NF-09) [NB500-446] - Staining of neurofilament medium protein in murine Neuro2A cells by antibody NF-09 conjugated with Dyomics 547 (red). DNA stained by Hoechst (blue).

Product Details

Summary
Reactivity Mu, Po, MaSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
NF-09
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

NF-M Antibody (NF-09) Summary

Immunogen
Pellet of pig brain cold stable proteins after depolymerization of microtubules.
Marker
Neuronal Marker
Specificity
The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form of medium neurofilament protein (160 kDa) of various species.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NEFM
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Immunocytochemistry - Carnoys fixative 2 x 3 min, blocking 1% glycine + 0.2% gelatin 10 min
Positive Control
NF-M Lysate (NBL1-13578)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for NF-M Antibody (NF-09)

  • NEF3
  • NEF3neurofilament medium polypeptide
  • NEFM
  • Neurofilament 3
  • neurofilament, medium polypeptide 150kDa
  • neurofilament, medium polypeptide
  • neurofilament-3 (150 kD medium)
  • NFM
  • NF-M
  • NF-M160 kDa neurofilament protein
  • NFMNeurofilament triplet M protein

Background

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NF-M Antibody (NB500-446) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB500-446AF405
Alexa Fluor 488 Labeled NB500-446AF488
Alexa Fluor 647 Labeled NB500-446AF647
Alexa Fluor 700 Labeled NB500-446AF700
DyLight 350 Labeled NB500-446UV
DyLight 405 Labeled NB500-446V
DyLight 488 Labeled NB500-446G
DyLight 680 Labeled NB500-446FR
DyLight 755 Labeled NB500-446IR

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Bioinformatics

Gene Symbol NEFM
Entrez