NF-L Antibody (2F11)

Product Discontinued

NF-L Antibody (2F11) Summary

• Immunogen: BALB/C mice were injected with purified neurofilaments from human brain.
• Localization: Cytoplasmic
• Marker: Neuronal Marker
• Specificity: This reacts with 70 and 200 kD component of the polypeptide subunit. It stains neurons, neuronal processes and peripheral nerves. This reacts with some neuroblastomas and gangliomas but not with other non-neuronal tumors.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NEFL
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:50
  • Western Blot 1:50-1:100

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for NF-L Antibody (2F11)

• 68 kDa neurofilament protein
• CMT2E
• NEFL
• neurofilament protein, light chain
• neurofilament subunit NF-L
• Neurofilament triplet L protein
• neurofilament, light polypeptide
• neurofilament-light
• NF68
• NF68FLJ53642
• NFL
• NF-L
• NFLlight polypeptide 68kDa

Background

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NF-L Antibody (NBP1-22555)(1)

Publication using NBP1-22555

• Puzzolo D, Pisani A, Malta C et al. Structural, ultrastructural, and morphometric study of the zebrafish ocular surface: a model for human corneal diseases Curr. Eye Res. 2017-11-07 [PMID: 29111817] (Human)

Bioinformatics

• Gene Symbol: NEFL