Recombinant Mouse Neuropoietin/NP Protein

SDS-Page: Recombinant Mouse Neuropoietin/NP Protein [NBP2-61439]

• Reactivity: Mu
• Applications: Bioactivity
• Concentration: LYOPH

Recombinant Mouse Neuropoietin/NP Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 182 amino acids corresponding to Neuropoietin/NP Source: E. coli

  Uniprot ID: P83714

  Amino Acid Sequence: APISPSEPIG QAYSLALYMQ KNTSALLQTY LQHQGSPFSD PGFSAPELQL STLPSAAVSF KTWHAMEDAE RLSRAQGAFL ALTQHLQLVG DDQSYLNPGS PILLAQLGAA RLRAQGLLGN MAAIMTALGL PIPPEEDTLG FVPFGASAFE RKCRGYIVTR EYGHWTDRAV RDLALLKAKY SA

• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 200 ng/ml, corresponding to a specific activity of > 5000 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: Ctf2
• Purity: >98%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of Neuropoietin/NP as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH8.0, 500 mM NaCl, with 0.5 mM DTT.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >98%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse Neuropoietin/NP Protein

• Cardiotrophin-2
• Ctf2
• CTF2P
• Gm494
• Neuropoietin
• NP

Background

Neuropoietin (NP; also known as cardiotrophin-2) is a 22 kDa member of the IL-6 family of cytokines. Considered to be the product of a gene duplication event involving cardiotrophin-1 (CT-1), NP helps to define a subfamily within the IL-6 family that includes CT-1, CLC and CTNF.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: Ctf2