Neuroplastin Antibody [Alexa Fluor® 488]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

Neuroplastin Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Neuroplastin
  Gln29-Pro338, predicted
  Accession # P97300
• Specificity: Detects mouse Neuroplastin in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human Neuroplastin-65 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroplastin Antibody [Alexa Fluor® 488]

• AW554172
• GP55
• GP55Stromal cell-derived receptor 1
• GP65SDR-1
• GP66
• MGC102805
• Neuroplastin
• np55
• np65
• NPTN
• SDFR1
• SDFR1DKFZp686L2477
• SDR1
• SDR1stromal cell derived factor receptor 1

Background

Neuroplastin (NPTN or neural plasticity-dependent synaptic rearrangement protein; also Np65 and SDR-1) is a 63-67 kDa member of the CD147 family, Ig‑superfamily of molecules. Its expression is primarily restricted to the PSD of select neurons, and it likely serves as a cell adhesion molecule between cells. NPTN is known to bind homotypically in trans, and this complex appears to interact with FGFR1 in cis. Engagement of the FGFR induces neurite outgrowth. Notably, rodent and human Np65 show distinctly different staining patterns in the hippocampus and cerebellum of the brain. Mature mouse NPTN is a 369 amino acid (aa) type I transmembrane glycoprotein. It possesses a 310 aa extracellular region (aa 29-338) that contains three Ig-like domains (aa 29-134; 148-234; 237-327), and a 38 aa cytoplasmic tail. There are three splice forms that have been described. One is a widely-expressed 53-57 kDa isoform (Np55) that contains a Glu substitution for aa 31‑147, a second is a splice form that shows the above substitution coupled to a deletion of aa 371-374, and a third is an isoform that contains a nine aa substitution for aa 1-235. Over aa 1-338, mouse NPTN shares 95% and 98% aa sequence identity with human and rat NPTN/Np65, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.