Neuroplastin Antibody

Western blot shows lysates of mouse brain (whole) tissue and mouse brain (cerebellum) tissue. PVDF membrane was probed with 0.05 µg/mL of Sheep Anti-Mouse Neuroplastin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7818) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuroplastin Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Neuroplastin
  Gln29-Pro338, predicted
  Accession # P97300
• Specificity: Detects mouse Neuroplastin in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant human Neuroplastin-65 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NPTN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroplastin Antibody

• AW554172
• GP55
• GP55Stromal cell-derived receptor 1
• GP65SDR-1
• GP66
• MGC102805
• Neuroplastin
• np55
• np65
• NPTN
• SDFR1
• SDFR1DKFZp686L2477
• SDR1
• SDR1stromal cell derived factor receptor 1

Background

Neuroplastin (NPTN or neural plasticity-dependent synaptic rearrangement protein; also Np65 and SDR-1) is a 63-67 kDa member of the CD147 family, Ig‑superfamily of molecules. Its expression is primarily restricted to the PSD of select neurons, and it likely serves as a cell adhesion molecule between cells. NPTN is known to bind homotypically in trans, and this complex appears to interact with FGFR1 in cis. Engagement of the FGFR induces neurite outgrowth. Notably, rodent and human Np65 show distinctly different staining patterns in the hippocampus and cerebellum of the brain. Mature mouse NPTN is a 369 amino acid (aa) type I transmembrane glycoprotein. It possesses a 310 aa extracellular region (aa 29-338) that contains three Ig-like domains (aa 29-134; 148-234; 237-327), and a 38 aa cytoplasmic tail. There are three splice forms that have been described. One is a widely-expressed 53-57 kDa isoform (Np55) that contains a Glu substitution for aa 31‑147, a second is a splice form that shows the above substitution coupled to a deletion of aa 371-374, and a third is an isoform that contains a nine aa substitution for aa 1-235. Over aa 1-338, mouse NPTN shares 95% and 98% aa sequence identity with human and rat NPTN/Np65, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuroplastin Antibody (AF7818)(7)

Publications using AF7818

• Hu, D;Wen, W;Li, H;Zhang, Z;Lin, H;Luo, J; Age-Related Alterations in the Expression of Mesencephalic Astrocyte-derived Neurotrophic Factor in the Brain and Their Impact on Neurobehavioral Functions bioRxiv : the preprint server for biology 2025-08-01 [PMID: 40766433] (Western Blot, Mouse)
• Jamwal, A;Constantin, CF;Hirschi, S;Henrich, S;Bildl, W;Fakler, B;Draper, SJ;Schulte, U;Higgins, MK; Erythrocyte invasion-neutralising antibodies prevent Plasmodium falciparum RH5 from binding to basigin-containing membrane protein complexes eLife 2023-10-05 [PMID: 37796723] (Affinity Purification, Human)
• Sherylanne Newton, Fanbo Kong, Adam J. Carlton, Carlos Aguilar, Andrew Parker, Gemma F. Codner et al. Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing PLOS Genetics 2022-01-31 [PMID: 35100259]
• Ilic K, Lin X, Malci A et al. Plasma Membrane Calcium ATPase-Neuroplastin Complexes Are Selectively Stabilized in GM1-Containing Lipid Rafts International Journal of Molecular Sciences 2021-12-18 [PMID: 34948386]
• Kuu Ikäheimo, Anni Herranen, Vilma Iivanainen, Tuuli Lankinen, Antti A Aarnisalo, Ville Sivonen et al. MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochlea Life Science Alliance 2022-02-01 [PMID: 34815294]
• T Wiwatpanit, SM Lorenzen, JA Cantú, CZ Foo, AK Hogan, F Márquez, JC Clancy, MJ Schipma, MA Cheatham, A Duggan, J García-Año Trans-differentiation of outer hair cells into inner hair cells in the absence of INSM1 Nature, 2018-10-10;0(0):. 2018-10-10 [PMID: 30305733] (IHC, Mouse)
• Neuroplastin Isoform Np55 Is Expressed in the Stereocilia of Outer Hair Cells and Required for Normal Outer Hair Cell Function J Neurosci, 2016-08-31;36(35):9201-16. 2016-08-31 [PMID: 27581460] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: NPTN
• Uniprot:
  • P97300(Mouse)