Neuroplastin Antibody [Unconjugated]

Western blot shows lysates of human brain (cerebellum, hypothalamus, and hippocampus) tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neuroplastin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neuroplastin was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 1.7 µg/mL Sheep Anti-Human Neuroplastin Isoform 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5174) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the dendrites of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuroplastin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Neuroplastin 55 isoform 1
  Gln29-Ser219
  Accession # Q9Y639.1
• Specificity: Detects human Neuroplastin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NPTN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroplastin Antibody [Unconjugated]

• AW554172
• GP55
• GP55Stromal cell-derived receptor 1
• GP65SDR-1
• GP66
• MGC102805
• Neuroplastin
• np55
• np65
• NPTN
• SDFR1
• SDFR1DKFZp686L2477
• SDR1
• SDR1stromal cell derived factor receptor 1

Background

Neuroplastin (NPTN; also stromal cell-derived receptor 1 and Np55) is a 52‑57 kDa member of the Ig-superfamily. It is widely expressed, and likely serves as a cell adhesion molecule in multiple tissues. Human Neuroplastin is 282 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains two Ig-like domains (aa 32‑119 and 122‑213) and a 38 aa cytoplasmic region (aa 245‑282). There is a brain-enriched 65 kDa form (Np65) that negatively regulates LTP in the hippocampus. It contains an N-terminal 116 aa Ig-like domain-containing insert after Asn30, and interacts homophilically. There is a third potential splice variant that shows an alternate start site at Met85 accompanied by a 23 aa substitution for aa 200‑282. Over aa 29‑229, human Neuroplastin is 91% aa identical to mouse Neuroplastin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuroplastin Antibody (AF5174)(1)

Publication using AF5174

• Leanne Carrott, Michael R. Bowl, Carlos Aguilar, Stuart L. Johnson, Lauren Chessum, Melissa West et al. Absence of Neuroplastin-65 Affects Synaptogenesis in Mouse Inner Hair Cells and Causes Profound Hearing Loss The Journal of Neuroscience 2016-01-06 [PMID: 26740663]

Bioinformatics

• Gene Symbol: NPTN
• Uniprot:
  • Q9Y639.1()