Neuroplastin Antibody (OTI3C4)

Western Blot: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NPTN monoclonal antibody.

Immunocytochemistry/Immunofluorescence: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NPTN.

Immunohistochemistry-Paraffin: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Staining of paraffin-embedded Human pancreas tissue using anti-NPTN mouse monoclonal antibody.

Western Blot: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NPTN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NPTN.

Immunohistochemistry-Paraffin: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NPTN mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NPTN mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NPTN mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NPTN mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neuroplastin Antibody (OTI3C4) [NBP2-01793] - Staining of paraffin-embedded Human liver tissue using anti-NPTN mouse monoclonal antibody.

• Reactivity: Hu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI3C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.65 mg/ml

Neuroplastin Antibody (OTI3C4) Summary

• Description: Novus Biologicals Mouse Neuroplastin Antibody (OTI3C4) (NBP2-01793) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Neuroplastin Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human NPTN(NP_059429) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPTN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 28.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.65 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neuroplastin Antibody (OTI3C4)

• AW554172
• GP55
• GP55Stromal cell-derived receptor 1
• GP65SDR-1
• GP66
• MGC102805
• Neuroplastin
• np55
• np65
• NPTN
• SDFR1
• SDFR1DKFZp686L2477
• SDR1
• SDR1stromal cell derived factor receptor 1

Background

This gene encodes a type I transmembrane protein belonging to the Ig superfamily. The protein is believed to be involved in cell-cell interactions or cell-substrate interactions. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuroplastin Antibody (NBP2-01793)(1)

Publication using NBP2-01793

• Li S, Wei X, Huang H et al. Neuroplastin exerts antiepileptic effects through binding to the ?1 subunit of GABA type A receptors to inhibit the internalization of the receptors Journal of translational medicine 2023-10-09 [PMID: 37814294] (WB, Rat)

FAQs for Neuroplastin Antibody (NBP2-01793). (Showing 1 - 1 of 1 FAQ).

1. Could you tell me the concentration of this antibody?
   • The concentration for the NBP2-01793 Lot # A001 is 0.65 mg/ml for 100 µl vial size.

Bioinformatics

• Gene Symbol: NPTN
• Entrez:
  • 27020(Human)