Neuroglycan C/CSPG5 Antibody [Unconjugated]

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Neuroglycan C/CSPG5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5685) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Neuroglycan C/CSPG5 at approximately 120 and 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Neuroglycan C/CSPG5 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Neuroglycan C/CSPG5
  Val31-Gln420
  Accession # AAQ04776
• Specificity: Detects human Neuroglycan C/CSPG5 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant mouse Neuroglycan C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CSPG5
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuroglycan C/CSPG5 Antibody [Unconjugated]

• CALEB
• chondroitin sulfate proteoglycan 5 (neuroglycan C)
• chondroitin sulfate proteoglycan 5
• CSPG5
• MGC44034
• Neuroglycan C
• NGC
• NGCAcidic leucine-rich EGF-like domain-containing brain protein

Background

Neuroglycan C (NGC; also CSPG5 and CALEB) is a 120‑150 kDa type I transmembrane glycoprotein and member of the neuregulin family of proteins (1, 2). Depending on its expression profile, NGC may be a glycoprotein of 120 kDa, or a chondroitin sulfate (CS) proteoglycan of 150 kDa (2, 3). Human NGC is synthesized as a 566 amino acid (aa) precursor that contains a 30 aa signal sequence, a 393 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 122 aa cytoplasmic region. The ECD contains one CS attachment domain (aa 34‑272), with CS attachment at Ser117, one EGF‑like domain (aa 371‑413), two potential sites for N‑linked glycosylation, and twelve potential sites for O‑linked glycosylation (4). Splicing variants produce three isoforms for human NGC. Isoform 1 is the long form. Isoform 2 has a deletion of aa 487‑513, while isoform 3 has an alternative start site at Met139 and the same deletion. Phosphorylation likely occurs at Ser249, and proteolysis generates a 75 kDa soluble fragment (5). Over aa 31‑420, human NGC shares 84% aa identity with mouse NGC. NGC is expressed in nervous tissue and is found on retinal ganglion cells, cerebellar Purkinje cells and hippocampal neurons (6). NGC may function as a growth and differentiation factor involved in neuritogenesis. One study shows that the recombinant ectodomain of NGC core protein enhances neurite outgrowth from rat neocortical neurons in culture via phosphatidylinositol 3‑kinase and protein kinase C signaling pathways (7). Another study states that NGC is a novel component of midkine receptors, a heparin‑binding growth factor that promotes cell attachment and process extension in oligodendroglial precursor‑like cells (3). NGC also acts as a growth factor by directly binding ERbB3 tyrosine kinase and transactivating ErbB2 (1).

1. Kinugasa, Y. et al. (2004) Biochem. Biophys. Res. Commun 321:1045.
2. Yasuda, Y. et al. (1998) Neurosci. Res. 32:313.
3. Ichihara-Tanaka, K. et al. (2006) J. Biol. Chem. 281:30857.
4. Aono, S. et al. (2004) J. Biol. Chem. 279:46536.
5. Shuo, T. et al. (2007) J. Neurochem. 102:1561.
6. Aono, S. et al. (2006) J. Neurosci. Res.83:110.
7. Nakanishi, K. et al. (2006) J. Biol. Chem. 281:24970.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CSPG5
• Uniprot:
  • AAQ04776()