Neuritin Antibody [Unconjugated]

Western blot shows lysates of human brain (cerebellum) tissue and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Rat Neuritin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF283) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuritin at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Product Discontinued

Neuritin Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neuritin
  Ala28-Gly114
  Accession # Q9NPD7
• Specificity: Detects human Neuritin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NRN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neuritin Antibody [Unconjugated]

• dJ380B8.2
• neuritin 1
• Neuritin
• NRN1
• NRNMGC44811

Background

Neuritin is a 14 kDa GPI-linked molecule that is expressed on developing and differentiating neurons as well as on hepatocytes. Neuritin is upregulated in response to BDNF, NT-3, androgens, and hypoxia, and it promotes neurite extension and arborization. Mature human Neuritin shares 99% and 100% aa sequence identity with mouse and rat Neuritin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neuritin Antibody (AF283)(5)

Publications using AF283

• C Xi, Y Zhang, M Yan, Q Lv, H Lu, J Zhou, Y Wang, J Li Exogenous neuritin treatment improves survivability and functions of Schwann cells with improved outgrowth of neurons in rat diabetic neuropathy J. Cell. Mol. Med., 2020-07-15;0(0):. 2020-07-15 [PMID: 32667138] (Western Blot, Rat)
• Tianxiang Huang, Lena H. Nguyen, Tiffany V. Lin, Xuan Gong, Longbo Zhang, Gi Bum Kim et al. In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons Neuroscience Research 2019-06-01 [PMID: 29857015] (Western Blot, Mouse)
• Jin-Jing Yao Neuritin Upregulates Kv4.2 ?-subunit of Potassium Channel Expression and Affects Neuronal Excitability by Regulating the Calcium-Calcineurin-NFATc4 Signaling Pathway J Biol Chem, 2016-06-15;0(0):. 2016-06-15 [PMID: 27307045] (Western Blot, Mouse)
• Choi , Yoori, Lee , Kihwan, Ryu , Junghwa, Kim , Hyoun Ge, Jeong , A Young, Woo , Ran-Sook, Lee , Jun-Ho, Hyun , Jin Won, Hahn , Seokyung, Kim , Joung-Hu, Kim , Hye-Sun Neuritin attenuates cognitive function impairments in tg2576 mouse model of Alzheimer's disease. PLoS ONE, 2014-08-07;9(8):e104121. 2014-08-07 [PMID: 25101829] (IHC-P, Human)
• Karamoysoyli E, Burnand RC, Tomlinson DR, Gardiner NJ Neuritin mediates nerve growth factor-induced axonal regeneration and is deficient in experimental diabetic neuropathy. Diabetes, 2007-10-01;57(1):181-9. 2007-10-01 [PMID: 17909094] (ICC, Western Blot, Rat)

Bioinformatics

• Gene Symbol: NRN1
• Uniprot:
  • Q9NPD7()